biostudies-literatureVillani AAssociazione Italiana per la Ricerca sul Cancroe71450https://www.ebi.ac.uk/biostudies/studies/S-EPMC12700705biostudies-literatureUnknown14(24)<h4>Background</h4>Pancreatic cancer (PC) remains one of the most lethal malignancies worldwide, characterized by late-stage diagnosis and a poor prognosis. This study explores the clinical, biochemical, and gut microbiota differences between PC patients and healthy controls (CTRL), as well as between subgroups of PC patients with pancreatic head cancer (PHC) and pancreatic body-tail cancer (PBTC).<h4>Methods</h4>A total of 72 PC patients and 37 CTRL subjects were included, with further stratification of PC patients into 45 PHC and 27 PBTC cases. Clinical and biochemical data were collected. Gut microbiota was analyzed by 16S rRNA gene sequencing. Alpha-diversity indices, Firmicutes/Bacteroidetes ratio and taxonomic composition were evaluated and compared in all the experimental group. CorCancer medicineComparative Analysis of Fecal Microbiota in Healthy Controls and Pancreatic Cancer Patients: A Focus on Tumor Localization Differences in Pancreatic Head and Body-Tail.PMC12700705IG 2019-ID. 23006 project - P.I. Pazienza ValerioVillani APanebianco CContu VMaiello EPazienza VMencarelli GBinda ECocomazzi GLatiano TPPerri FVirgili EfalseComparative Analysis of Fecal Microbiota in Healthy Controls and Pancreatic Cancer Patients: A Focus on Tumor Localization Differences in Pancreatic Head and Body-Tail.<h4>Background</h4>Pancreatic cancer (PC) remains one of the most lethal malignancies worldwide, characterized by late-stage diagnosis and a poor prognosis. This study explores the clinical, biochemical, and gut microbiota differences between PC patients and healthy controls (CTRL), as well as between subgroups of PC patients with pancreatic head cancer (PHC) and pancreatic body-tail cancer (PBTC).<h4>Methods</h4>A total of 72 PC patients and 37 CTRL subjects were included, with further stratification of PC patients into 45 PHC and 27 PBTC cases. Clinical and biochemical data were collected. Gut microbiota was analyzed by 16S rRNA gene sequencing. Alpha-diversity indices, Firmicutes/Bacteroidetes ratio and taxonomic composition were evaluated and compared in all the experimental group. Cor2025-01-01T00:00:00Z2025 Dec2026-06-06T02:54:58.997Z2026-05-24T03:12:59.783ZS-EPMC127007054138628510.1002/cam4.71450