biostudies-literatureUnknown43(12)Luo JEfficient and accurate nanocarrier development for targeted drug delivery is hindered by a lack of methods to analyze its cell-level biodistribution across whole organisms. Here we present Single Cell Precision Nanocarrier Identification (SCP-Nano), an integrated experimental and deep learning pipeline to comprehensively quantify the targeting of nanocarriers throughout the whole mouse body at single-cell resolution. SCP-Nano reveals the tissue distribution patterns of lipid nanoparticles (LNPs) after different injection routes at doses as low as 0.0005 mg kg^-1-far below the detection limits of conventional whole body imaging techniques. We demonstrate that intramuscularly injected LNPs carrying SARS-CoV-2 spike mRNA reach heart tissue, leading to proteome changes, suggesting imNature biotechnology2009-2022https://www.ebi.ac.uk/biostudies/studies/S-EPMC12700832biostudies-literatureNanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning.PMC12700832Todorov MIKadletz KBruns OTHoeher LAl-Maskari RCalandra GMSu JGalensowske NJeridi DNegwer MSingh IZhao SMinde DPDietz HGroschup BKimna CSimats AOhn TLChen YBhatia HSSingh VKhalin IPaetzold JProtzer UHorvath IErturk AUssar SPlesnila NAli MRong ZMolbay MElsner MArus BALuo JPan CKick BHellal FRoth SLiesz AZeidler RfalseNanocarrier imaging at single-cell resolution across entire mouse bodies with deep learning.Efficient and accurate nanocarrier development for targeted drug delivery is hindered by a lack of methods to analyze its cell-level biodistribution across whole organisms. Here we present Single Cell Precision Nanocarrier Identification (SCP-Nano), an integrated experimental and deep learning pipeline to comprehensively quantify the targeting of nanocarriers throughout the whole mouse body at single-cell resolution. SCP-Nano reveals the tissue distribution patterns of lipid nanoparticles (LNPs) after different injection routes at doses as low as 0.0005 mg kg^-1-far below the detection limits of conventional whole body imaging techniques. We demonstrate that intramuscularly injected LNPs carrying SARS-CoV-2 spike mRNA reach heart tissue, leading to proteome changes, suggesting im2025-01-01T00:00:00Z2025 Dec2026-06-06T02:07:02.755Z2026-05-24T03:12:21.266ZS-EPMC127008323980993310.1038/s41587-024-02528-1