biostudies-literatureUnknown109(1)Costa LFRThe growing demand for sustainable agricultural pest control solutions has increased the use of microbial bioinput. This study characterized an industrial bioinput produced by Saccharopolyspora spinosa, focusing on identifying metabolites and quantifying spinosyns A and D by HPLC-MS. The concentrations of these compounds were 29.4 ± 3.6 mg L^-1 and 13.3 ± 1.5 mg L^-1, respectively, for a total of 42.7 mg L^-1. The limit of quantification (LOQ) and limit of detection (LOD) values obtained were 13.7 ng L^-1 and 4.16 ng L^-1 for spinosyn A and 7.63 ng L^-1 and 2.31 ng L^-1 for spinosyn D, respectively. The bioinput contained essential amino acids (leucine, phenylalanine, and tryptophan), plant cell death inducers (sphinganiApplied microbiology and biotechnology267https://www.ebi.ac.uk/biostudies/studies/S-EPMC12701044biostudies-literatureChemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa.PMC12701044Maia FMDos Santos MPMartins IPSMartins MMAlvarenga CDde Abreu Delvaux Junior NRodrigues LPMiranda AEPereira MCTDos Anjos JLSCosta LFRda Costa Silva TSilva JFfalseChemical composition and bioinsecticidal activity of bioinputs produced by Saccharopolyspora spinosa.The growing demand for sustainable agricultural pest control solutions has increased the use of microbial bioinput. This study characterized an industrial bioinput produced by Saccharopolyspora spinosa, focusing on identifying metabolites and quantifying spinosyns A and D by HPLC-MS. The concentrations of these compounds were 29.4 ± 3.6 mg L^-1 and 13.3 ± 1.5 mg L^-1, respectively, for a total of 42.7 mg L^-1. The limit of quantification (LOQ) and limit of detection (LOD) values obtained were 13.7 ng L^-1 and 4.16 ng L^-1 for spinosyn A and 7.63 ng L^-1 and 2.31 ng L^-1 for spinosyn D, respectively. The bioinput contained essential amino acids (leucine, phenylalanine, and tryptophan), plant cell death inducers (sphingani2025-01-01T00:00:00Z2025 Dec2026-06-14T06:04:43.177Z2026-06-14T03:16:40.913ZS-EPMC127010444138193710.1007/s00253-025-13655-3