{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Auvray M"],"funding":["NIGMS NIH HHS","Max-Planck-Gesellschaft"],"pagination":["11107"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12701065"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Fluorescent probes enable precise visualization of dynamic cellular processes, especially when combined with super-resolution imaging techniques that overcome the diffraction limit. However, traditional labeling strategies, including fluorescent protein fusions (e.g., GFP) or ligand-linked fluorophores, often perturb protein function or induce biological side effects. Here, we report a covalent fluorescent probe for endogenous tubulin, a key cytoskeletal protein governing cell division, motility, and intracellular transport. Using cabazitaxel as a tubulin targeting moiety and silicon-rhodamine as a cell permeable fluorophore, we designed and optimized probe, 6-SiR-o-C<sub>9</sub>-CTX, containing a biocompatible cleavable linker with a sulfonium center. It exhibits cell permeability, fluoro"],"journal":["Nature communications"],"pubmed_title":["Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells."],"pmcid":["PMC12701065"],"funding_grant_id":["R01 GM129325"],"pubmed_authors":["Auvray M","Dybkov O","Lukinavicius G","Urlaub H","Koenen T"],"additional_accession":[]},"is_claimable":false,"name":"Biocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cells.","description":"Fluorescent probes enable precise visualization of dynamic cellular processes, especially when combined with super-resolution imaging techniques that overcome the diffraction limit. However, traditional labeling strategies, including fluorescent protein fusions (e.g., GFP) or ligand-linked fluorophores, often perturb protein function or induce biological side effects. Here, we report a covalent fluorescent probe for endogenous tubulin, a key cytoskeletal protein governing cell division, motility, and intracellular transport. Using cabazitaxel as a tubulin targeting moiety and silicon-rhodamine as a cell permeable fluorophore, we designed and optimized probe, 6-SiR-o-C<sub>9</sub>-CTX, containing a biocompatible cleavable linker with a sulfonium center. It exhibits cell permeability, fluoro","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T02:59:55.966Z","creation":"2026-05-25T03:07:53.304Z"},"accession":"S-EPMC12701065","cross_references":{"pubmed":["41381485"],"doi":["10.1038/s41467-025-67319-x"]}}