{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Roig-Soriano X"],"funding":["Generalitat de Catalunya","Fundació Bosch i Gimpera","European Regional Development Fund"],"pagination":["163-176"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12701751"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["59(1)"],"pubmed_abstract":["<h4>Aim</h4>To characterise and evaluate mucoadhesive strength and antibacterial properties of a nanotechnological formulation for endodontic disinfection based on biodegradable nanoparticles dispersed in a thermosensitive gel containing calcium hydroxide (Ca(OH)<sub>2</sub>-NPs-gel).<h4>Methodology</h4>Morphology of Ca(OH)<sub>2</sub>-NPs-gel was studied using transmission electron microscopy. Moreover, Ca(OH)<sub>2</sub>-NPs-gel was sterilised using gamma irradiation (25 kGy), and the stability after the sterilisation process was studied by measuring Ca(OH)<sub>2</sub>-NPs-gel average size, polydispersity index, zeta potential and encapsulation efficiency. To assess the ex vivo mucoadhesive strength, extracted single-rooted human teeth were used to measure the force necessary to separate the formulation from the teeth. In addition, the short-time stability of Ca(OH)<sub>2</sub>-NPs-gel was evaluated monthly, analysing entrapment efficacy, backscattering and transmittance of Ca(OH)<sub>2</sub>-NPs-gel stored at different temperatures (4°C, 25°C and 37°C). Furthermore, the antibacterial analysis of Ca(OH)<sub>2</sub>-NPs-gel was performed against Enterococcus faecalis inoculated in extracted human single-root teeth and evaluated by confocal and scanning electron microscopy. Finally, the metabolic activity of bacteria was studied through a resazurin assay to evaluate bacterial survival after treatment.<h4>Results</h4>Ca(OH)<sub>2</sub>-NPs-gel owned a round shape and a smooth surface without particle aggregation. Sterilisation did not induce an alteration in Ca(OH)<sub>2</sub>-NPs-gel physicochemical properties and Ca(OH)<sub>2</sub>-NPs-gel presented a high adhesion strength. In addition, 4°C was the best temperature to store Ca(OH)<sub>2</sub>-NPs-gel. Regarding the antibacterial therapeutic efficacy, Ca(OH)<sub>2</sub>-NPs-gel possesses suitable antibacterial properties, indicating that it efficiently reduces bacterial biofilms.<h4>Conclusion</h4>Calcium hydroxide-loaded PLGA nanoparticles dispersed in a thermosensitive gel have been developed, optimised and characterised, obtaining excellent antibacterial properties and achieving bacterial disinfection levels similar to those of commercial formulations."],"journal":["International endodontic journal"],"pubmed_title":["A Thermosensitive Gel Containing Biodegradable Nanoparticles Carrying Calcium Hydroxide as Antibacterial Intracanal Therapy."],"pmcid":["PMC12701751"],"funding_grant_id":["FBG 600324","PROD00141","2022 FISDU 00360"],"pubmed_authors":["Espina M","Roig-Soriano X","Duran-Sindreu F","Teule M","Sanchez JAG","Sanchez-Lopez E","Garcia ML","Delgado LM","Elmsmari F","Esteruelas G"],"additional_accession":[]},"is_claimable":false,"name":"A Thermosensitive Gel Containing Biodegradable Nanoparticles Carrying Calcium Hydroxide as Antibacterial Intracanal Therapy.","description":"<h4>Aim</h4>To characterise and evaluate mucoadhesive strength and antibacterial properties of a nanotechnological formulation for endodontic disinfection based on biodegradable nanoparticles dispersed in a thermosensitive gel containing calcium hydroxide (Ca(OH)<sub>2</sub>-NPs-gel).<h4>Methodology</h4>Morphology of Ca(OH)<sub>2</sub>-NPs-gel was studied using transmission electron microscopy. Moreover, Ca(OH)<sub>2</sub>-NPs-gel was sterilised using gamma irradiation (25 kGy), and the stability after the sterilisation process was studied by measuring Ca(OH)<sub>2</sub>-NPs-gel average size, polydispersity index, zeta potential and encapsulation efficiency. To assess the ex vivo mucoadhesive strength, extracted single-rooted human teeth were used to measure the force necessary to separate the formulation from the teeth. In addition, the short-time stability of Ca(OH)<sub>2</sub>-NPs-gel was evaluated monthly, analysing entrapment efficacy, backscattering and transmittance of Ca(OH)<sub>2</sub>-NPs-gel stored at different temperatures (4°C, 25°C and 37°C). Furthermore, the antibacterial analysis of Ca(OH)<sub>2</sub>-NPs-gel was performed against Enterococcus faecalis inoculated in extracted human single-root teeth and evaluated by confocal and scanning electron microscopy. Finally, the metabolic activity of bacteria was studied through a resazurin assay to evaluate bacterial survival after treatment.<h4>Results</h4>Ca(OH)<sub>2</sub>-NPs-gel owned a round shape and a smooth surface without particle aggregation. Sterilisation did not induce an alteration in Ca(OH)<sub>2</sub>-NPs-gel physicochemical properties and Ca(OH)<sub>2</sub>-NPs-gel presented a high adhesion strength. In addition, 4°C was the best temperature to store Ca(OH)<sub>2</sub>-NPs-gel. Regarding the antibacterial therapeutic efficacy, Ca(OH)<sub>2</sub>-NPs-gel possesses suitable antibacterial properties, indicating that it efficiently reduces bacterial biofilms.<h4>Conclusion</h4>Calcium hydroxide-loaded PLGA nanoparticles dispersed in a thermosensitive gel have been developed, optimised and characterised, obtaining excellent antibacterial properties and achieving bacterial disinfection levels similar to those of commercial formulations.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-06T02:57:05.373Z","creation":"2026-05-24T03:13:05.836Z"},"accession":"S-EPMC12701751","cross_references":{"pubmed":["41054854"],"doi":["10.1111/iej.70032"]}}