biostudies-literatureUnknown23(1)Yang YF<h4>Background</h4>Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the attachment of glycosylphosphatidylinositol (GPI) anchors to membrane proteins implicated in oncogenic signaling. However, the specific contribution of individual GPI-T subunits to head and neck cancer (HNC) remains unclear.<h4>Methods</h4>We first compare the expression profiles of GPI-T subunits in HNC and then integrate multi-omics analyses to assess <i>phosphatidylinositol glycan class K</i> (<i>PIGK</i>) expression, genomic alterations, function and pathway enrichment, molecular interactions, and immune associations. Clinical relevance is validated by immunohistochemistry on tissue microarray, and <i>in vitro</i> assays were conducted to assess PIGK-mediated phenotypes, regulation of <i>Family with sequeInternational journal of medical sciences161-176https://www.ebi.ac.uk/biostudies/studies/S-EPMC12701967biostudies-literatureIntegrative analysis identifies PIGK as an oncogenic glycosylphosphatidylinositol transamidase subunit with prognostic, immunological, and therapeutic relevance in head and neck cancer.PMC12701967Chou CYYang YFLin YHYu PLLiao JBfalseIntegrative analysis identifies PIGK as an oncogenic glycosylphosphatidylinositol transamidase subunit with prognostic, immunological, and therapeutic relevance in head and neck cancer.<h4>Background</h4>Glycosylphosphatidylinositol transamidase (GPI-T) catalyzes the attachment of glycosylphosphatidylinositol (GPI) anchors to membrane proteins implicated in oncogenic signaling. However, the specific contribution of individual GPI-T subunits to head and neck cancer (HNC) remains unclear.<h4>Methods</h4>We first compare the expression profiles of GPI-T subunits in HNC and then integrate multi-omics analyses to assess <i>phosphatidylinositol glycan class K</i> (<i>PIGK</i>) expression, genomic alterations, function and pathway enrichment, molecular interactions, and immune associations. Clinical relevance is validated by immunohistochemistry on tissue microarray, and <i>in vitro</i> assays were conducted to assess PIGK-mediated phenotypes, regulation of <i>Family with seque2026-01-01T00:00:00Z20262026-06-06T08:58:44.592Z2026-05-28T03:12:06.81ZS-EPMC127019674139937110.7150/ijms.121484