{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kajino H"],"funding":["National Institute of Neurological Disorders and Stroke","Agilent Technologies Japan Ltd","Japan Agency for Medical Research and Development","NINDS NIH HHS","National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["130422"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12704479"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["130"],"pubmed_abstract":["Rhodamines are useful fluorescent molecules for activity-based sensing. One powerful design strategy is to exploit changes in the open/closed equilibrium of rhodamine amides. In the context of amide derivatives of rhodamine B, a prototypical member of the rhodamine family, this strategy has been especially useful in the development of activity-based indicators for protons and metal ions. This is because at neutral pH, the closed form of rhodamine B amides dominates, making this otherwise bright and fluorescent dye non-fluorescent. At acidic pH, the equilibrium favors the open form. Despite a wealth of methods to trigger Lewis acid-mediated fluorogenicity of rhodamine B amides, there are far fewer ways to shift the open-close equilibrium to favor the open form at neutral pH. Here, we demons"],"journal":["Bioorganic & medicinal chemistry letters"],"pubmed_title":["Fluorogenic rhodamine B derivatives that become brighter at neutral pH."],"pmcid":["PMC12704479"],"funding_grant_id":["T32GM152983","R01 NS098088","T32 GM152983","R01NS098088"],"pubmed_authors":["Kwon JH","Miller EW","Kajino H"],"additional_accession":[]},"is_claimable":false,"name":"Fluorogenic rhodamine B derivatives that become brighter at neutral pH.","description":"Rhodamines are useful fluorescent molecules for activity-based sensing. One powerful design strategy is to exploit changes in the open/closed equilibrium of rhodamine amides. In the context of amide derivatives of rhodamine B, a prototypical member of the rhodamine family, this strategy has been especially useful in the development of activity-based indicators for protons and metal ions. This is because at neutral pH, the closed form of rhodamine B amides dominates, making this otherwise bright and fluorescent dye non-fluorescent. At acidic pH, the equilibrium favors the open form. Despite a wealth of methods to trigger Lewis acid-mediated fluorogenicity of rhodamine B amides, there are far fewer ways to shift the open-close equilibrium to favor the open form at neutral pH. Here, we demons","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-06T03:41:45.639Z","creation":"2026-05-25T03:08:03.675Z"},"accession":"S-EPMC12704479","cross_references":{"pubmed":["41033359"],"doi":["10.1016/j.bmcl.2025.130422"]}}