{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Paudel S"],"funding":["NICHD NIH HHS","North Carolina State University","Agriculture and Food Research Initiative Competitive","U.S. Department of Agriculture National Institute of Food and Agriculture","North Carolina Agricultural Foundation","National Institutes of Health","NIH HHS"],"pagination":["1475-1489"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12706476"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["113(6)"],"pubmed_abstract":["In ungulates such as pigs and sheep, blastocyst elongation is essential for implantation, driven by coordinated trophoblast proliferation, cytoskeletal remodeling, and nutrient signaling. L-arginine (Arg), a conditionally essential amino acid enriched in uterine histotroph during early pregnancy, plays a key role in conceptus development; however, its genomic effects on the trophectoderm are poorly defined. Therefore, we investigated Arg-responsive transcriptomic changes in porcine (pTr2) and ovine (oTr1) trophectoderm cells using RNA-seq and integrative pathway analyses. Arg significantly stimulated proliferation in both cell types in a non-linear, dose-dependent manner, with maximal effects at 0.2 mM, consistent with physiological levels. Transcriptomic profiling identified 2723 and 5369"],"journal":["Biology of reproduction"],"pubmed_title":["Uterine histotroph and conceptus development. V. Comparative analyses of arginine-mediated transcriptomic reprogramming in conceptus trophectoderm of pigs and sheep†."],"pmcid":["PMC12706476"],"funding_grant_id":["R03HD112581","R01 HD111463","2022-67015-36491","R03 HD112581","665143","R01HD111463","665163"],"pubmed_authors":["Peng X","Burghardt RC","Bazer FW","Hu G","Wang T","Paudel S","Wang X","Yu H"],"additional_accession":[]},"is_claimable":false,"name":"Uterine histotroph and conceptus development. V. Comparative analyses of arginine-mediated transcriptomic reprogramming in conceptus trophectoderm of pigs and sheep†.","description":"In ungulates such as pigs and sheep, blastocyst elongation is essential for implantation, driven by coordinated trophoblast proliferation, cytoskeletal remodeling, and nutrient signaling. L-arginine (Arg), a conditionally essential amino acid enriched in uterine histotroph during early pregnancy, plays a key role in conceptus development; however, its genomic effects on the trophectoderm are poorly defined. Therefore, we investigated Arg-responsive transcriptomic changes in porcine (pTr2) and ovine (oTr1) trophectoderm cells using RNA-seq and integrative pathway analyses. Arg significantly stimulated proliferation in both cell types in a non-linear, dose-dependent manner, with maximal effects at 0.2 mM, consistent with physiological levels. Transcriptomic profiling identified 2723 and 5369","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-02T03:14:29.244Z","creation":"2026-06-02T03:10:29.973Z"},"accession":"S-EPMC12706476","cross_references":{"pubmed":["41055590"],"doi":["10.1093/biolre/ioaf226"]}}