biostudies-literaturePaudel SNICHD NIH HHSNorth Carolina State UniversityAgriculture and Food Research Initiative CompetitiveU.S. Department of Agriculture National Institute of Food and AgricultureNorth Carolina Agricultural FoundationNational Institutes of HealthNIH HHS1475-1489https://www.ebi.ac.uk/biostudies/studies/S-EPMC12706476biostudies-literatureUnknown113(6)In ungulates such as pigs and sheep, blastocyst elongation is essential for implantation, driven by coordinated trophoblast proliferation, cytoskeletal remodeling, and nutrient signaling. L-arginine (Arg), a conditionally essential amino acid enriched in uterine histotroph during early pregnancy, plays a key role in conceptus development; however, its genomic effects on the trophectoderm are poorly defined. Therefore, we investigated Arg-responsive transcriptomic changes in porcine (pTr2) and ovine (oTr1) trophectoderm cells using RNA-seq and integrative pathway analyses. Arg significantly stimulated proliferation in both cell types in a non-linear, dose-dependent manner, with maximal effects at 0.2 mM, consistent with physiological levels. Transcriptomic profiling identified 2723 and 5369Biology of reproductionUterine histotroph and conceptus development. V. Comparative analyses of arginine-mediated transcriptomic reprogramming in conceptus trophectoderm of pigs and sheep†.PMC12706476R03HD112581R01 HD1114632022-67015-36491R03 HD112581665143R01HD111463665163Peng XBurghardt RCBazer FWHu GWang TPaudel SWang XYu HfalseUterine histotroph and conceptus development. V. Comparative analyses of arginine-mediated transcriptomic reprogramming in conceptus trophectoderm of pigs and sheep†.In ungulates such as pigs and sheep, blastocyst elongation is essential for implantation, driven by coordinated trophoblast proliferation, cytoskeletal remodeling, and nutrient signaling. L-arginine (Arg), a conditionally essential amino acid enriched in uterine histotroph during early pregnancy, plays a key role in conceptus development; however, its genomic effects on the trophectoderm are poorly defined. Therefore, we investigated Arg-responsive transcriptomic changes in porcine (pTr2) and ovine (oTr1) trophectoderm cells using RNA-seq and integrative pathway analyses. Arg significantly stimulated proliferation in both cell types in a non-linear, dose-dependent manner, with maximal effects at 0.2 mM, consistent with physiological levels. Transcriptomic profiling identified 2723 and 53692025-01-01T00:00:00Z2025 Dec2026-06-02T03:14:29.244Z2026-06-02T03:10:29.973ZS-EPMC127064764105559010.1093/biolre/ioaf226