biostudies-literatureUnknown135(12)Tadikonda AIUSM Department of Otolaryngology-HNS<h4>Objective</h4>Unilateral vocal fold paralysis (UVFP) due to recurrent laryngeal nerve injury (RLN) is a major cause of voice disorders. We have recently identified three biomolecules (agrin, acetylcholine, and neuregulin) with the potential to promote reinnervation after RLN injury. This study aimed to determine if a gelatin injectable with the reinnervating biomolecules will induce site-specific amplification of neurotrophic factor release and reinnervation after RLN injury in unilateral vocal fold paralysis.<h4>Methods</h4>C57BL/6 mice underwent RLN transection with the following treatment allocations: saline control (N = 16), biomolecule cocktail only (N = 16), and biomolecule-infused gelatin (N = 16). All injectables were delivered into the denervated thyroarytenoid muscle. AssessmThe Laryngoscope4781-4792https://www.ebi.ac.uk/biostudies/studies/S-EPMC12706572biostudies-literatureNovel Biomolecule-Infused Gelatin Injectable for Treatment of Recurrent Laryngeal Nerve Injury.PMC12706572Tadikonda AWesson THalum SFinnegan PRJackson CAnekal SChen LWSobczak GLiu JCEgan MAfalseNovel Biomolecule-Infused Gelatin Injectable for Treatment of Recurrent Laryngeal Nerve Injury.<h4>Objective</h4>Unilateral vocal fold paralysis (UVFP) due to recurrent laryngeal nerve injury (RLN) is a major cause of voice disorders. We have recently identified three biomolecules (agrin, acetylcholine, and neuregulin) with the potential to promote reinnervation after RLN injury. This study aimed to determine if a gelatin injectable with the reinnervating biomolecules will induce site-specific amplification of neurotrophic factor release and reinnervation after RLN injury in unilateral vocal fold paralysis.<h4>Methods</h4>C57BL/6 mice underwent RLN transection with the following treatment allocations: saline control (N = 16), biomolecule cocktail only (N = 16), and biomolecule-infused gelatin (N = 16). All injectables were delivered into the denervated thyroarytenoid muscle. Assessm2025-01-01T00:00:00Z2025 Dec2026-06-06T03:52:34.715Z2026-05-25T03:12:13.562ZS-EPMC127065724095206510.1002/lary.32459