biostudies-literatureAssadipapari MWolfson FoundationUK-India Education and Research InitiativeScheme for Promotion of Academic and Research CollaborationRoyal SocietyBiotechnology and Biological Sciences Research CouncilAcademy of Medical SciencesHORIZON EUROPE Marie Sklodowska-Curie Actions1045-1054https://www.ebi.ac.uk/biostudies/studies/S-EPMC12706752biostudies-literatureUnknown18(2)Ferritin is a multimeric protein that stores and releases iron, emerging as a promising candidate for nanomedicine, particularly in targeted drug delivery. Its ability to disassemble and reassemble under specific environments is crucial for encapsulating and releasing therapeutic payloads. In this work, we monitor ferritin reassembly and disassembly in real time at the single-molecule level using polymer-coated solid-state nanopores. The coating enabled continuous ion current recording of ferritin fragment translocations for up to 1 hour without clogging, overcoming a major challenge of uncoated nanopores. This long-term recording allowed us to track the full reassembly and disassembly processes. We show that while traditional population-based analysis can identify the presence of fragmentNanoscaleTracking single-molecule ferritin reassembly and disassembly using polymer-coated nanopores.PMC12706752BB/T0083690/1Springboard Award (SBF0010\1008)I4WORLDBB/T000627/1Xu LLi JAdam MRahmani MAssadipapari MClark AWKara HHudson AJSantana Vega MYing CYousefi AHuang JAZargarbashi SSoleimanian AWills MFKfalseTracking single-molecule ferritin reassembly and disassembly using polymer-coated nanopores.Ferritin is a multimeric protein that stores and releases iron, emerging as a promising candidate for nanomedicine, particularly in targeted drug delivery. Its ability to disassemble and reassemble under specific environments is crucial for encapsulating and releasing therapeutic payloads. In this work, we monitor ferritin reassembly and disassembly in real time at the single-molecule level using polymer-coated solid-state nanopores. The coating enabled continuous ion current recording of ferritin fragment translocations for up to 1 hour without clogging, overcoming a major challenge of uncoated nanopores. This long-term recording allowed us to track the full reassembly and disassembly processes. We show that while traditional population-based analysis can identify the presence of fragment2026-01-01T00:00:00Z2026 Jan2026-07-15T06:39:13.701Z2026-06-30T03:20:17.272ZS-EPMC127067524139987210.1039/d5nr02885j