{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Verma N"],"funding":["European Research Council"],"pagination":["13565-13570"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12706835"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(49)"],"pubmed_abstract":["Marine polysaccharides, including β3-xylan and mixed-linkage xylan (MLX), play a central role in the ocean's carbon cycle. Using an optimized xylose donor, automated glycan assembly enabled the synthesis of β3- and MLX-oligosaccharides up to decasaccharides. Interrogation of these structurally defined substrates with the marine xylanases Xyl4, AlXyn26A, and MfXyn26A revealed a surprisingly broad substrate scope of MLXases toward both β3-xylan and MLX motifs, highlighting AGA-derived glycans as powerful probes for studying marine polysaccharide metabolism."],"journal":["Organic letters"],"pubmed_title":["Automated Syntheses of Xylan Oligosaccharides Containing β3-Linkages Enable Substrate Specificity Studies of Xylanases from Marine Bacteria."],"pmcid":["PMC12706835"],"funding_grant_id":["101087063"],"pubmed_authors":["Pfrengle F","Rustmeier N","Osswald U","Verma N"],"additional_accession":[]},"is_claimable":false,"name":"Automated Syntheses of Xylan Oligosaccharides Containing β3-Linkages Enable Substrate Specificity Studies of Xylanases from Marine Bacteria.","description":"Marine polysaccharides, including β3-xylan and mixed-linkage xylan (MLX), play a central role in the ocean's carbon cycle. Using an optimized xylose donor, automated glycan assembly enabled the synthesis of β3- and MLX-oligosaccharides up to decasaccharides. Interrogation of these structurally defined substrates with the marine xylanases Xyl4, AlXyn26A, and MfXyn26A revealed a surprisingly broad substrate scope of MLXases toward both β3-xylan and MLX motifs, highlighting AGA-derived glycans as powerful probes for studying marine polysaccharide metabolism.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T03:59:31.837Z","creation":"2026-05-25T03:11:56.428Z"},"accession":"S-EPMC12706835","cross_references":{"pubmed":["41296978"],"doi":["10.1021/acs.orglett.5c04433"]}}