{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Juanpere-Borras M"],"funding":["Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, Indonesia","China Scholarship Council","European Union’s Horizon 2020 research and innovation program"],"pagination":["2600145"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12707516"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["<i>Streptococcus suis</i> is a major cause of sepsis and meningitis in pigs, and zoonosis through the emergence of disease-associated lineages. The ability of <i>S. suis</i> to adapt and survive in host environments, such as blood and cerebrospinal fluid (CSF), is important for pathogenesis. Here, we used Transposon Sequencing (Tn-seq) coupled with Nanopore sequencing to identify conditionally essential genes (CEGs) for the growth of <i>S. suis</i> P1/7 in active porcine serum (APS) and CSF derived from choroid plexus organoids. To our knowledge, this is the first successful application of ONT to Tn-library screening, enabling rapid local runs and a publicly available analysis pipeline. Through comparative fitness analyses, we identified 33 CEGs that support growth in APS and 25 CEGs in CS"],"journal":["Virulence"],"pubmed_title":["Genome-wide Identification of conditionally essential genes supporting &lt;i&gt;Streptococcus suis&lt;/i&gt; growth in serum and cerebrospinal fluid."],"pmcid":["PMC12707516"],"funding_grant_id":["201906350084","956154"],"pubmed_authors":["Fernandez-Ciruelos B","Wells JM","Arifa N","Boekhorst J","Zhao T","Wagenaar T","van Baarlen P","Juanpere-Borras M","Sanyal R"],"additional_accession":[]},"is_claimable":false,"name":"Genome-wide Identification of conditionally essential genes supporting &lt;i&gt;Streptococcus suis&lt;/i&gt; growth in serum and cerebrospinal fluid.","description":"<i>Streptococcus suis</i> is a major cause of sepsis and meningitis in pigs, and zoonosis through the emergence of disease-associated lineages. The ability of <i>S. suis</i> to adapt and survive in host environments, such as blood and cerebrospinal fluid (CSF), is important for pathogenesis. Here, we used Transposon Sequencing (Tn-seq) coupled with Nanopore sequencing to identify conditionally essential genes (CEGs) for the growth of <i>S. suis</i> P1/7 in active porcine serum (APS) and CSF derived from choroid plexus organoids. To our knowledge, this is the first successful application of ONT to Tn-library screening, enabling rapid local runs and a publicly available analysis pipeline. Through comparative fitness analyses, we identified 33 CEGs that support growth in APS and 25 CEGs in CS","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Dec","modification":"2026-06-06T04:03:01.163Z","creation":"2026-05-25T03:11:59.348Z"},"accession":"S-EPMC12707516","cross_references":{"pubmed":["41396007"],"doi":["10.1080/21505594.2025.2600145"]}}