biostudies-literatureDong WMinistry of Science and Technology of the People's Republic of China295https://www.ebi.ac.uk/biostudies/studies/S-EPMC12709716biostudies-literatureUnknown18(1)<h4>Background</h4>Hydroxyurea (HU) is an antitumor drug. However, HU exposure is associated with diminished ovarian reserve (DOR). Zi Chong granules, a Chinese Medicine, can protect against DOR, but little is known regarding its underlying mechanisms of DOR treatment, and thus the target of the present study.<h4>Methods</h4>Seventy-two female Kunming (KM) mice (4-6 weeks old) were randomly divided into three groups: the control group (Con), the hydroxyurea group (HU), and the Zi Chong group (ZC). The Con group received saline, while the HU and ZC groups were administered hydroxyurea (400 mg/kg/d) by gavage for 21 days to induce diminished ovarian reserve (DOR). Subsequently, the Con and HU groups were given saline, while the ZC group was treated with Zi Chong granules (2.72 g/kg/d) for 15Journal of ovarian researchTherapeutic mechanisms of Zi Chong granules against hydroxyurea-induced diminished ovarian reserve based on integrated multi-omics analyses.PMC127097162018YFC1704305Liu YDong WLiu ZWan QLu HGuo XXie LLiu SChen RfalseTherapeutic mechanisms of Zi Chong granules against hydroxyurea-induced diminished ovarian reserve based on integrated multi-omics analyses.<h4>Background</h4>Hydroxyurea (HU) is an antitumor drug. However, HU exposure is associated with diminished ovarian reserve (DOR). Zi Chong granules, a Chinese Medicine, can protect against DOR, but little is known regarding its underlying mechanisms of DOR treatment, and thus the target of the present study.<h4>Methods</h4>Seventy-two female Kunming (KM) mice (4-6 weeks old) were randomly divided into three groups: the control group (Con), the hydroxyurea group (HU), and the Zi Chong group (ZC). The Con group received saline, while the HU and ZC groups were administered hydroxyurea (400 mg/kg/d) by gavage for 21 days to induce diminished ovarian reserve (DOR). Subsequently, the Con and HU groups were given saline, while the ZC group was treated with Zi Chong granules (2.72 g/kg/d) for 152025-01-01T00:00:00Z2025 Dec2026-06-06T04:16:55.769Z2026-05-25T03:11:51.04ZS-EPMC127097164140285410.1186/s13048-025-01846-5