biostudies-literatureUnknown120Burgueno-Rodriguez G<h4>Background</h4>The conformation of the Sordida subcomplex has been a topic of prolonged debate, with diverse methodological approaches employed to discern its constituent species. Up to now, Triatoma sordida, T. garciabesi and T. rosai comprise part of this subcomplex. Distinguishing and identifying these three species pose significant challenges due to their pronounced morphological similarity, overlapping distributions, and presence of natural hybrids.<h4>Objectives</h4>This study aims to uncover the genetic diversity and geographic spread of these three species.<h4>Methods</h4>We analysed a mitochondrial cytochrome b gene fragment and complemented it with chromosomal studies across natural populations from an extensive geographical range, including Argentina, Bolivia, Brazil, and PaMemorias do Instituto Oswaldo Cruze250088https://www.ebi.ac.uk/biostudies/studies/S-EPMC12711208biostudies-literatureReconstructing Sordida subcomplex (Hemiptera, Reduviidae, Triatominae) phylogeny across species distribution rangeFIRST REVIEW ROUND - REVIEWERS COMMENTSAUTHORS RESPONSE TO REVIEWERSREVIEWERS COMMENTS.PMC12711208Rios NLopes CMPanzera FLorite PVega-Gomez MCCavallo MJRodriguez CSNattero JRojas de Arias ABurgueno-Rodriguez GLobbia PASansoni-Ruidiaz BARolon MCarbajal-de-la-Fuente ALPita SPiccinali RVfalseReconstructing Sordida subcomplex (Hemiptera, Reduviidae, Triatominae) phylogeny across species distribution rangeFIRST REVIEW ROUND - REVIEWERS COMMENTSAUTHORS RESPONSE TO REVIEWERSREVIEWERS COMMENTS.<h4>Background</h4>The conformation of the Sordida subcomplex has been a topic of prolonged debate, with diverse methodological approaches employed to discern its constituent species. Up to now, Triatoma sordida, T. garciabesi and T. rosai comprise part of this subcomplex. Distinguishing and identifying these three species pose significant challenges due to their pronounced morphological similarity, overlapping distributions, and presence of natural hybrids.<h4>Objectives</h4>This study aims to uncover the genetic diversity and geographic spread of these three species.<h4>Methods</h4>We analysed a mitochondrial cytochrome b gene fragment and complemented it with chromosomal studies across natural populations from an extensive geographical range, including Argentina, Bolivia, Brazil, and Pa2025-01-01T00:00:00Z20252026-06-07T05:25:59.63Z2026-06-07T03:07:09.559ZS-EPMC127112084141735410.1590/0074-02760250088