biostudies-literatureHaljasmagi LEesti TeadusagentuurEuropean Union through HORIZON Coordination and Support Actionse70100https://www.ebi.ac.uk/biostudies/studies/S-EPMC12712876biostudies-literatureUnknown55(12)Selecting a sensitive test system is crucial to measure IFNα in serum samples. Simoa digital ELISA stands out for its high sensitivity and strong correlation with transcript-based IFN-induced gene (ISG) scores. In SLE, the ISG score is explained by circulating IFNα levels with negligible contribution from IFNβ or IFNγ. Created in BioRender.European journal of immunologyUltrasensitive Test Systems Are Required to Quantify Interferon Alpha Protein in Serum Samples.PMC12712876101159920PRG1117Duffy DKisand KPeterson PMeisalu SHaljasmagi LBondet VfalseUltrasensitive Test Systems Are Required to Quantify Interferon Alpha Protein in Serum Samples.Selecting a sensitive test system is crucial to measure IFNα in serum samples. Simoa digital ELISA stands out for its high sensitivity and strong correlation with transcript-based IFN-induced gene (ISG) scores. In SLE, the ISG score is explained by circulating IFNα levels with negligible contribution from IFNβ or IFNγ. Created in BioRender.2025-01-01T00:00:00Z2025 Dec2026-06-06T05:07:58.534Z2026-05-26T03:12:11.68ZS-EPMC127128764141016910.1002/eji.70100