biostudies-literatureShi YFoundation of the Agricultural Resources and Environmental Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Arable Land ConservationGuangxi Key Technologies R&D ProgramNational Natural Science Foundation of ChinaGuangxi Science and Technology Major Programe10577https://www.ebi.ac.uk/biostudies/studies/S-EPMC12713033biostudies-literatureUnknown12(47)By regulating cellulose molecular weight through enzymatic hydrolysis to obtain different carbon precursors, three types of carbon quantum dots (CQDs) are synthesized via hydrothermal methods: cellulose enzyme-hydrolyzed solids-based carbon quantum dots (CES-CQDs), cellulose enzyme-hydrolyzed mixtures-based carbon quantum dots (CEM-CQDs), and cellulose hydrothermal degradation products-based carbon quantum dots (CHD-CQDs). By controlling the molecular weight of the cellulose precursor, the O/C ratio of the CQDs is systematically modulated from 0.25 to 0.61, resulting in a more than five-fold increase in fluorescence intensity and an approximately seven-fold improvement in quantum yield (QY). Density functional theory (DFT) calculations indicate that high O/C ratio enhances oscillator strenAdvanced science (Weinheim, Baden-Wurttemberg, Germany)O/C Ratio-Driven Fluorescence Enhancement in Cellulose-Derived Carbon Quantum Dots: Mechanistic Insights into Reaction Pathways.PMC12713033AB250691422268007AB25069140AA22117013No.23-026-12-24KF1andNo.23-026-12-25KF02Chong MNKang XZhao SZhang ZChen XOu XSong XShi YfalseO/C Ratio-Driven Fluorescence Enhancement in Cellulose-Derived Carbon Quantum Dots: Mechanistic Insights into Reaction Pathways.By regulating cellulose molecular weight through enzymatic hydrolysis to obtain different carbon precursors, three types of carbon quantum dots (CQDs) are synthesized via hydrothermal methods: cellulose enzyme-hydrolyzed solids-based carbon quantum dots (CES-CQDs), cellulose enzyme-hydrolyzed mixtures-based carbon quantum dots (CEM-CQDs), and cellulose hydrothermal degradation products-based carbon quantum dots (CHD-CQDs). By controlling the molecular weight of the cellulose precursor, the O/C ratio of the CQDs is systematically modulated from 0.25 to 0.61, resulting in a more than five-fold increase in fluorescence intensity and an approximately seven-fold improvement in quantum yield (QY). Density functional theory (DFT) calculations indicate that high O/C ratio enhances oscillator stren2025-01-01T00:00:00Z2025 Dec2026-06-06T04:27:20.573Z2026-05-25T03:11:54.585ZS-EPMC127130334105136810.1002/advs.202510577