{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Reilly SP"],"funding":["National Institutes of Health"],"pagination":["eaea8707"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12716397"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(51)"],"pubmed_abstract":["Nonclassical CD8<sup>+</sup> T cells can compensate for classical CD8<sup>+</sup> T cell effector responses during murine cytomegalovirus (MCMV) infection. Through a combination of motif-based discovery, predictive algorithms, AlphaFold3 structural modeling, and biological assays, we identified multiple MCMV and human cytomegalovirus (HCMV) peptides that bind to Qa-1 and HLA-E, respectively. In the mouse system, we demonstrated that these virally encoded antigens stimulate Qa-1-restricted CD8<sup>+</sup> T cells ex vivo, which can be tracked using MCMV peptide-loaded Qa-1 tetramers. Adoptive transfer of predominantly Qa-1 tetramer<sup>+</sup> CD8<sup>+</sup> T cells into RAG-1-deficient mice protects them from mortality, underscoring the critical role of these cells in host defense. Single"],"journal":["Science advances"],"pubmed_title":["Unconventional CD8&lt;sup&gt;+&lt;/sup&gt; T cell surveillance of cytomegalovirus via Qa-1/HLA-E-restricted epitope recognition."],"pmcid":["PMC12716397"],"funding_grant_id":["T32HL134625","R01AI173163","F31DE032593","R24AI072073","R01AI046709","3R01AI046709-19S1"],"pubmed_authors":["Fugere C","Smith ML","Demers D","Hogan MJ","Zemmour D","Borys SM","Brossay L","Reilly SP"],"additional_accession":[]},"is_claimable":false,"name":"Unconventional CD8&lt;sup&gt;+&lt;/sup&gt; T cell surveillance of cytomegalovirus via Qa-1/HLA-E-restricted epitope recognition.","description":"Nonclassical CD8<sup>+</sup> T cells can compensate for classical CD8<sup>+</sup> T cell effector responses during murine cytomegalovirus (MCMV) infection. Through a combination of motif-based discovery, predictive algorithms, AlphaFold3 structural modeling, and biological assays, we identified multiple MCMV and human cytomegalovirus (HCMV) peptides that bind to Qa-1 and HLA-E, respectively. In the mouse system, we demonstrated that these virally encoded antigens stimulate Qa-1-restricted CD8<sup>+</sup> T cells ex vivo, which can be tracked using MCMV peptide-loaded Qa-1 tetramers. Adoptive transfer of predominantly Qa-1 tetramer<sup>+</sup> CD8<sup>+</sup> T cells into RAG-1-deficient mice protects them from mortality, underscoring the critical role of these cells in host defense. Single","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-08T05:32:44.336Z","creation":"2026-06-08T03:09:03.32Z"},"accession":"S-EPMC12716397","cross_references":{"pubmed":["41417899"],"doi":["10.1126/sciadv.aea8707"]}}