biostudies-literatureUnknown35Liu XChoroidal neovascularization (CNV), characterized by abnormal vessel growth and vascular leakage, is the hallmark of wet age-related macular degeneration (wAMD) and a leading cause of irreversible vision loss. Although anti-vascular endothelial growth factor (VEGF) therapies remain the current standard, their frequent administration and limited long-term efficacy highlight the need for novel treatments. Here, we developed a miR-22-3p-loaded tetrahedral framework nucleic acids (tFNAs-miR22) nanostructure and evaluated its efficacy in CNV suppression. The nanocomplex was structurally validated, exhibiting high assembly fidelity and superior intraocular stability compared to serum conditions. In a laser-induced CNV mouse model, a single intravitreal injection of tFNAs-miR22 significantly reduMaterials today. Bio102578https://www.ebi.ac.uk/biostudies/studies/S-EPMC12719782biostudies-literatureSustained suppression of choroidal neovascularization by intraocularly stable tetrahedral network encapsulated miR-22-3p.PMC12719782Liu XCai TDing XWang QLi SHuang JChen LYu GChen YWang YLuo DfalseSustained suppression of choroidal neovascularization by intraocularly stable tetrahedral network encapsulated miR-22-3p.Choroidal neovascularization (CNV), characterized by abnormal vessel growth and vascular leakage, is the hallmark of wet age-related macular degeneration (wAMD) and a leading cause of irreversible vision loss. Although anti-vascular endothelial growth factor (VEGF) therapies remain the current standard, their frequent administration and limited long-term efficacy highlight the need for novel treatments. Here, we developed a miR-22-3p-loaded tetrahedral framework nucleic acids (tFNAs-miR22) nanostructure and evaluated its efficacy in CNV suppression. The nanocomplex was structurally validated, exhibiting high assembly fidelity and superior intraocular stability compared to serum conditions. In a laser-induced CNV mouse model, a single intravitreal injection of tFNAs-miR22 significantly redu2025-01-01T00:00:00Z2025 Dec2026-06-06T05:27:41.385Z2026-05-26T03:12:05.497ZS-EPMC127197824143870810.1016/j.mtbio.2025.102578