biostudies-literatureShin YSMinistry of Science and ICT, South KoreaNational Research Foundation of KoreaSeoul National University114056https://www.ebi.ac.uk/biostudies/studies/S-EPMC12719785biostudies-literatureUnknown28(12)As the primary interface between the growing root and the soil, the root tip experiences continuous mechanical and environmental stress. The root cap protects this region through dynamic turnover, in which outer cells are regularly shed and replaced by new cells derived from inner initials. This turnover proceeds via two distinct pathways: programmed cell death in the lateral root cap and detachment of the outermost columella root cap. Focusing on the latter, we show that reactive oxygen species (ROS) levels oscillate within the columella during its developmental cycle in <i>Arabidopsis thaliana</i>. The disruption of these oscillations through chemical inhibitors or exogenous ROS impairs columella initial division and detachment. We identify RbohC and RbohE as major ROS producers, negativiScienceAn RbohC and RbohE-mediated ROS oscillatory circuit regulates root cap turnover in Arabidopsis.PMC12719785RS-2023-NR076388RS-2021-NR60084Jeon WTJung HShin YSLee YDo THTfalseAn RbohC and RbohE-mediated ROS oscillatory circuit regulates root cap turnover in Arabidopsis.As the primary interface between the growing root and the soil, the root tip experiences continuous mechanical and environmental stress. The root cap protects this region through dynamic turnover, in which outer cells are regularly shed and replaced by new cells derived from inner initials. This turnover proceeds via two distinct pathways: programmed cell death in the lateral root cap and detachment of the outermost columella root cap. Focusing on the latter, we show that reactive oxygen species (ROS) levels oscillate within the columella during its developmental cycle in <i>Arabidopsis thaliana</i>. The disruption of these oscillations through chemical inhibitors or exogenous ROS impairs columella initial division and detachment. We identify RbohC and RbohE as major ROS producers, negativ2025-01-01T00:00:00Z2025 Dec2026-06-09T04:56:39.092Z2026-06-09T03:08:01.724ZS-EPMC127197854143806910.1016/j.isci.2025.114056