biostudies-literatureXiong TInnovative Research Group Project of the National Natural Science Foundation of ChinaXi&apos;an Agricultural Technology Research General ProjectXi'an Agricultural Technology Research General ProjectNational Key Research and Development Program of China177https://www.ebi.ac.uk/biostudies/studies/S-EPMC12720437biostudies-literatureUnknown16(1)<h4>Background</h4>Goat milk is increasingly recognized for high digestibility and a distinctive compositional profile. Protein acetylation, an important post-translational modification, regulates biosynthetic and metabolic pathways. This study aimed to identify critical acetylated proteins and specific modification sites involved in milk production and component synthesis in dairy goats, thereby elucidating the molecular mechanisms of lactation. We performed a comparative TMT-based acetylomic and proteomic analysis of mammary tissues from Saanen dairy goats during peak lactation and the dry period using LC-MS/MS. A candidate acetylation site was further investigated in goat mammary epithelial cells (GMECs) through site-directed mutagenesis and lipid metabolic assays, establishing functionJournal of animal science and biotechnologyBDH1 acetylation at K116 modulates milk fat production in dairy goats.PMC127204373170209824NYGG00252022YFF1000102Tian MLi CLuo JDeng LXiong TChen CHan CGong XLoor JJfalseBDH1 acetylation at K116 modulates milk fat production in dairy goats.<h4>Background</h4>Goat milk is increasingly recognized for high digestibility and a distinctive compositional profile. Protein acetylation, an important post-translational modification, regulates biosynthetic and metabolic pathways. This study aimed to identify critical acetylated proteins and specific modification sites involved in milk production and component synthesis in dairy goats, thereby elucidating the molecular mechanisms of lactation. We performed a comparative TMT-based acetylomic and proteomic analysis of mammary tissues from Saanen dairy goats during peak lactation and the dry period using LC-MS/MS. A candidate acetylation site was further investigated in goat mammary epithelial cells (GMECs) through site-directed mutagenesis and lipid metabolic assays, establishing function2025-01-01T00:00:00Z2025 Dec2026-06-06T04:56:40.447Z2026-05-26T03:11:59.979ZS-EPMC127204374142365310.1186/s40104-025-01315-5