biostudies-literatureUnknown126Tian LHigh-altitude lakes in Tibet represent unique and underexplored reservoirs of microbial diversity. An investigation of yeast diversity in Basom Lake, a high-altitude lake in Tibet, China, led to the discovery of two novel species: <i>Microbotryozyma lacustris</i> <b>sp. nov.</b> and <i>Cyberlindnera basumtsoensis</i> <b>sp. nov.</b> Phylogenetic analyses of the ITS region and D1/D2 LSU rRNA gene sequences, complemented by phenotypic characterization, confirmed their distinct taxonomic status. <i>Microbotryozyma lacustris</i> represents the third species described in its genus and the first documented occurrence of <i>Microbotryozyma</i> in a freshwater habitat. <i>Cyberlindnera basumtsoensis</i> further expands the ecological diversity of the genus <i>Cyberlindnera</i>. This study significantly enriches the taxonomic framework of both genera and underscores the value of high-altitude lakes as reservoirs of novel yeast diversity.MycoKeys135-150https://www.ebi.ac.uk/biostudies/studies/S-EPMC12723391biostudies-literature&lt;i&gt;Microbotryozyma lacustris&lt;/i&gt; sp. nov. (Basidiomycota, Ustilentylomataceae) and &lt;i&gt;Cyberlindnera basumtsoensis&lt;/i&gt; sp. nov. (Ascomycota, Phaffomycetaceae), two novel yeasts isolated from freshwater Lake Basom Tso, China.PMC12723391Tian LZheng YYPhurbu Dfalse&lt;i&gt;Microbotryozyma lacustris&lt;/i&gt; sp. nov. (Basidiomycota, Ustilentylomataceae) and &lt;i&gt;Cyberlindnera basumtsoensis&lt;/i&gt; sp. nov. (Ascomycota, Phaffomycetaceae), two novel yeasts isolated from freshwater Lake Basom Tso, China.High-altitude lakes in Tibet represent unique and underexplored reservoirs of microbial diversity. An investigation of yeast diversity in Basom Lake, a high-altitude lake in Tibet, China, led to the discovery of two novel species: <i>Microbotryozyma lacustris</i> <b>sp. nov.</b> and <i>Cyberlindnera basumtsoensis</i> <b>sp. nov.</b> Phylogenetic analyses of the ITS region and D1/D2 LSU rRNA gene sequences, complemented by phenotypic characterization, confirmed their distinct taxonomic status. <i>Microbotryozyma lacustris</i> represents the third species described in its genus and the first documented occurrence of <i>Microbotryozyma</i> in a freshwater habitat. <i>Cyberlindnera basumtsoensis</i> further expands the ecological diversity of the genus <i>Cyberlindnera</i>. This study significantly enriches the taxonomic framework of both genera and underscores the value of high-altitude lakes as reservoirs of novel yeast diversity.2025-01-01T00:00:00Z20252026-06-06T05:09:24.344Z2026-05-26T03:12:15.81ZS-EPMC127233914144690310.3897/mycokeys.126.173807