biostudies-literatureGulum LBolu Abant İzzet Baysal University1471https://www.ebi.ac.uk/biostudies/studies/S-EPMC12730045biostudies-literatureUnknown14(12)<i>Ganoderma lucidum</i> has long been recognized for its medicinal properties, particularly due to its antioxidant, anti-inflammatory, and pro-apoptotic components such as polysaccharides and triterpenoids. This study aimed to evaluate the cytotoxic and molecular effects of ethanol and methanol extracts of <i>G. lucidum</i> as well as doxorubicin on MCF-7 breast cancer cells. The cytotoxicity was assessed via MTT assay. The methanol extract showed stronger cytotoxicity (IC<sub>50</sub>: 62.37 µg/mL) than the ethanol extract, while doxorubicin exhibited an IC<sub>50</sub> value of 0.66 mM. Phenolic profiling by HPLC revealed high levels of vanillic acid, gallic acid and (-)-epicatechin in the methanol extract, while volatile compounds such as hexanal and acetic acid were identified by GC-MAntioxidants (Basel, Switzerland)Evaluation of Anticancer Potential of <i>Ganoderma lucidum</i> on MCF-7 Breast Cancer Cells Through Genetic Transcription of Energy Metabolism.PMC127300452024-BDP-6.10.41-0008Guler ECapkınoglu EGulum LCelik ABTutar YfalseEvaluation of Anticancer Potential of <i>Ganoderma lucidum</i> on MCF-7 Breast Cancer Cells Through Genetic Transcription of Energy Metabolism.<i>Ganoderma lucidum</i> has long been recognized for its medicinal properties, particularly due to its antioxidant, anti-inflammatory, and pro-apoptotic components such as polysaccharides and triterpenoids. This study aimed to evaluate the cytotoxic and molecular effects of ethanol and methanol extracts of <i>G. lucidum</i> as well as doxorubicin on MCF-7 breast cancer cells. The cytotoxicity was assessed via MTT assay. The methanol extract showed stronger cytotoxicity (IC<sub>50</sub>: 62.37 µg/mL) than the ethanol extract, while doxorubicin exhibited an IC<sub>50</sub> value of 0.66 mM. Phenolic profiling by HPLC revealed high levels of vanillic acid, gallic acid and (-)-epicatechin in the methanol extract, while volatile compounds such as hexanal and acetic acid were identified by GC-M2025-01-01T00:00:00Z2025 Dec2026-05-28T03:15:06.313Z2026-05-28T03:08:01.877ZS-EPMC127300454146267110.3390/antiox14121471