{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bianchi M"],"funding":["European Union - Next Generation EU within the National Recovery and Resilience Plan (PNRR) THE-Tuscany Health Ecosystem, Spoke 7-Innovating Translational Medicine-Subproject 5-Innovative models for management of infections caused by antibiotic-resistant","SFI Research Infrastructure Call 2012"],"pagination":["1271"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12730115"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(12)"],"pubmed_abstract":["<b>Background/Objectives:</b> Postbiotics derived from lactic acid bacteria are emerging as promising antimicrobial agents due to their antibacterial, antibiofilm, and immunomodulatory properties. Among their metabolites, lactic acid (LA) is thought to play a major role in antimicrobial activity. This study investigated the proteomic response of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> to <i>Lacticaseibacillus rhamnosus</i> cell-free supernatant (CFS) and compared it with that elicited by LA alone. <b>Methods:</b> Overnight bacterial cultures were exposed to sub-MIC LA or CFS (1:10 for <i>P. aeruginosa</i> and 1:8 for <i>S. aureus</i>; ~12.5-15.6 mM LA) for 6 h at 37 °C. Intracellular proteins were harvested and subsequently quantified and purified to be analysed by H"],"journal":["Antibiotics (Basel, Switzerland)"],"pubmed_title":["Proteomic Analysis of the Differential Response of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; and &lt;i&gt;Staphylococcus aureus&lt;/i&gt; to &lt;i&gt;Lacticaseibacillus rhamnosus&lt;/i&gt; Cell-Free Supernatant and Lactic Acid."],"pmcid":["PMC12730115"],"funding_grant_id":["Project code: ECS00000017; CUP I53C22000780001","12/RI/2346 (3)"],"pubmed_authors":["Esin S","Kavanagh K","Batoni G","Bianchi M","Maisetta G"],"additional_accession":[]},"is_claimable":false,"name":"Proteomic Analysis of the Differential Response of &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; and &lt;i&gt;Staphylococcus aureus&lt;/i&gt; to &lt;i&gt;Lacticaseibacillus rhamnosus&lt;/i&gt; Cell-Free Supernatant and Lactic Acid.","description":"<b>Background/Objectives:</b> Postbiotics derived from lactic acid bacteria are emerging as promising antimicrobial agents due to their antibacterial, antibiofilm, and immunomodulatory properties. Among their metabolites, lactic acid (LA) is thought to play a major role in antimicrobial activity. This study investigated the proteomic response of <i>Pseudomonas aeruginosa</i> and <i>Staphylococcus aureus</i> to <i>Lacticaseibacillus rhamnosus</i> cell-free supernatant (CFS) and compared it with that elicited by LA alone. <b>Methods:</b> Overnight bacterial cultures were exposed to sub-MIC LA or CFS (1:10 for <i>P. aeruginosa</i> and 1:8 for <i>S. aureus</i>; ~12.5-15.6 mM LA) for 6 h at 37 °C. Intracellular proteins were harvested and subsequently quantified and purified to be analysed by H","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-05-28T03:21:11.96Z","creation":"2026-05-28T03:11:27.503Z"},"accession":"S-EPMC12730115","cross_references":{"pubmed":["41463773"],"doi":["10.3390/antibiotics14121271"]}}