biostudies-literatureUnknown13Kerroum FMedicinal plants constitute a valuable natural resource of bioactive phytochemicals, which are increasingly studied for their therapeutic potential and broad applications in the pharmaceutical, nutraceutical, and cosmetic fields. <i>Rheum officinale</i>, a medicinal rhubarb species, is appreciated for the presence of biologically active compounds with therapeutic relevance. This work analyses the chemical composition, including the phytochemical profile, and pharmacological activities of <i>Rheum officinale</i> Baill. stems in Algeria. The plant extract was analyzed for its notable antioxidant capacity using various assays, including DPPH, ABTS, β-carotene bleaching, ferric and also cupric reducing power, and metal chelation. The inhibitory potential against cholinesterase and α-amylase waFrontiers in chemistry1661223https://www.ebi.ac.uk/biostudies/studies/S-EPMC12730168biostudies-literature&lt;i&gt;Rheum officinale&lt;/i&gt; Baill.: chemical characterization and &lt;i&gt;in-vitro&lt;/i&gt; biological activities.PMC12730168Kerroum FAtoki AVCakir OAtoui AYilmaz MAKhattabi LMessaoudi Mfalse&lt;i&gt;Rheum officinale&lt;/i&gt; Baill.: chemical characterization and &lt;i&gt;in-vitro&lt;/i&gt; biological activities.Medicinal plants constitute a valuable natural resource of bioactive phytochemicals, which are increasingly studied for their therapeutic potential and broad applications in the pharmaceutical, nutraceutical, and cosmetic fields. <i>Rheum officinale</i>, a medicinal rhubarb species, is appreciated for the presence of biologically active compounds with therapeutic relevance. This work analyses the chemical composition, including the phytochemical profile, and pharmacological activities of <i>Rheum officinale</i> Baill. stems in Algeria. The plant extract was analyzed for its notable antioxidant capacity using various assays, including DPPH, ABTS, β-carotene bleaching, ferric and also cupric reducing power, and metal chelation. The inhibitory potential against cholinesterase and α-amylase wa2025-01-01T00:00:00Z20252026-06-06T04:37:56.948Z2026-05-25T03:12:10.949ZS-EPMC127301684145102510.3389/fchem.2025.1661223