{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(12)"],"submitter":["Alexandre D"],"pubmed_abstract":["<b>Background/Objectives</b>: Late diagnosis hampers effective treatment of non-small cell lung cancer (NSCLC). This study evaluated whether circulating microRNAs (miRs), miR-155 and miR-3196, measured in liquid biopsy peripheral blood mononuclear cells (PBMCs), can serve as potential non-invasive biomarkers for NSCLC diagnosis, patient stratification, therapy monitoring, and prognosis. <b>Methods</b>: RNA was isolated from PBMCs of 136 NSCLC patients and 64 healthy donors. RT-qPCR quantified miR expression in PBMCs after predefined QC filtering: miR-155-3p (NSCLC <i>n</i> = 63; controls <i>n</i> = 28), miR-3196 (NSCLC <i>n</i> = 55; controls <i>n</i> = 28), and miR-155-5p (NSCLC <i>n</i> = 23; controls <i>n</i> = 12). Diagnostic performance was assessed using receiver operating characteristic (ROC) analyses, reporting area under the curve (AUC), and threshold-dependent sensitivity/specificity. Survival was analyzed with Kaplan-Meier/Cox methods. Associations with clinicopathological variables (stage, metastasis, smoking, EGFR, and KRAS status), treatment response (chemotherapy, immunotherapy, TKIs), and survival outcomes were examined. <b>Results</b>: miR-155-3p was upregulated in NSCLC, whereas miR-3196 was downregulated relative to controls; AUCs were 0.881 and 0.784, respectively. At high-sensitivity operating points, specificity was lower (≈29-30%), consistent with PBMC miRs reflecting both immune activation and tumor burden. In adenocarcinoma, miR-155-3p was associated with advanced stage, metastatic disease and smoking history. miR-3196 aligned with features of metastatic progression. During systemic therapy (chemotherapy, immunotherapy, TKIs), circulating levels of both miRs tended to normalize. Notably, normalization of miR-155-3p levels was associated with improved overall survival, supporting its prognostic value and utility for treatment monitoring. <b>Conclusions</b>: Circulating miR-155-3p and miR-3196 in PBMCs are promising screening/monitoring non-invasive candidates rather than stand-alone NSCLC diagnostics at current thresholds. Combining these miRs with additional biomarkers and/or clinical covariates and tuning decision thresholds may enhance specificity for diagnostic use. While preliminary, these findings warrant validation in large, prospective studies with standardized protocols to enable clinical implementation."],"journal":["Biomedicines"],"pagination":["2946"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12730197"],"repository":["biostudies-literature"],"pubmed_title":["miRNA-155-3p and miRNA-3196 as Potential Biomarkers in Liquid Biopsies of Non-Small Cell Lung Cancer Patients."],"pmcid":["PMC12730197"],"pubmed_authors":["Valente S","Alexandre D","Baptista PV","Polido J","Rocha DP","Cruz C","Fernandes AR"],"additional_accession":[]},"is_claimable":false,"name":"miRNA-155-3p and miRNA-3196 as Potential Biomarkers in Liquid Biopsies of Non-Small Cell Lung Cancer Patients.","description":"<b>Background/Objectives</b>: Late diagnosis hampers effective treatment of non-small cell lung cancer (NSCLC). This study evaluated whether circulating microRNAs (miRs), miR-155 and miR-3196, measured in liquid biopsy peripheral blood mononuclear cells (PBMCs), can serve as potential non-invasive biomarkers for NSCLC diagnosis, patient stratification, therapy monitoring, and prognosis. <b>Methods</b>: RNA was isolated from PBMCs of 136 NSCLC patients and 64 healthy donors. RT-qPCR quantified miR expression in PBMCs after predefined QC filtering: miR-155-3p (NSCLC <i>n</i> = 63; controls <i>n</i> = 28), miR-3196 (NSCLC <i>n</i> = 55; controls <i>n</i> = 28), and miR-155-5p (NSCLC <i>n</i> = 23; controls <i>n</i> = 12). Diagnostic performance was assessed using receiver operating characteristic (ROC) analyses, reporting area under the curve (AUC), and threshold-dependent sensitivity/specificity. Survival was analyzed with Kaplan-Meier/Cox methods. Associations with clinicopathological variables (stage, metastasis, smoking, EGFR, and KRAS status), treatment response (chemotherapy, immunotherapy, TKIs), and survival outcomes were examined. <b>Results</b>: miR-155-3p was upregulated in NSCLC, whereas miR-3196 was downregulated relative to controls; AUCs were 0.881 and 0.784, respectively. At high-sensitivity operating points, specificity was lower (≈29-30%), consistent with PBMC miRs reflecting both immune activation and tumor burden. In adenocarcinoma, miR-155-3p was associated with advanced stage, metastatic disease and smoking history. miR-3196 aligned with features of metastatic progression. During systemic therapy (chemotherapy, immunotherapy, TKIs), circulating levels of both miRs tended to normalize. Notably, normalization of miR-155-3p levels was associated with improved overall survival, supporting its prognostic value and utility for treatment monitoring. <b>Conclusions</b>: Circulating miR-155-3p and miR-3196 in PBMCs are promising screening/monitoring non-invasive candidates rather than stand-alone NSCLC diagnostics at current thresholds. Combining these miRs with additional biomarkers and/or clinical covariates and tuning decision thresholds may enhance specificity for diagnostic use. While preliminary, these findings warrant validation in large, prospective studies with standardized protocols to enable clinical implementation.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-28T03:16:05.383Z","creation":"2026-05-28T03:07:23.884Z"},"accession":"S-EPMC12730197","cross_references":{"pubmed":["41462957"],"doi":["10.3390/biomedicines13122946"]}}