biostudies-literatureReinsberg AEstonian Research Council1286https://www.ebi.ac.uk/biostudies/studies/S-EPMC12730293biostudies-literatureUnknown15(12)<b>Objective</b>: Neuronal growth regulator 1 (<i>Negr1</i>) is a GPI-anchored neuronal cell adhesion molecule of the IgLON superfamily associated with multiple psychiatric disorders. This study aimed to investigate behavioral and molecular adaptations to social isolation (SI) stress in <i>Negr1</i>-deficient (<i>Negr1^-/-</i>) mice. <b>Methods</b>: Male and female <i>Negr1^-/-</i> and wild-type (Wt) mice (n = 10 per group) were exposed to two weeks of SI or group housing (Ctl). Behavioral assays assessed exploratory and anxiety-like behavior. Gene expression analyses in the prefrontal cortex and hippocampus were performed using RT-qPCR, focusing on GABAergic, neurotrophic, and IgLON family genes. <b>Results</b>: SI-induced weight loss in <i>Negr1^-/-</i> miceBrain sciencesNegr1 Deficiency Modulates Sex-Specific Neurobehavioral Adaptations to Social Isolation.PMC12730293PRG2544 and TARISTU24-TK22Singh KJayaram MPhilips MAReinsberg AVasar EMikheim KfalseNegr1 Deficiency Modulates Sex-Specific Neurobehavioral Adaptations to Social Isolation.<b>Objective</b>: Neuronal growth regulator 1 (<i>Negr1</i>) is a GPI-anchored neuronal cell adhesion molecule of the IgLON superfamily associated with multiple psychiatric disorders. This study aimed to investigate behavioral and molecular adaptations to social isolation (SI) stress in <i>Negr1</i>-deficient (<i>Negr1^-/-</i>) mice. <b>Methods</b>: Male and female <i>Negr1^-/-</i> and wild-type (Wt) mice (n = 10 per group) were exposed to two weeks of SI or group housing (Ctl). Behavioral assays assessed exploratory and anxiety-like behavior. Gene expression analyses in the prefrontal cortex and hippocampus were performed using RT-qPCR, focusing on GABAergic, neurotrophic, and IgLON family genes. <b>Results</b>: SI-induced weight loss in <i>Negr1^-/-</i> mice2025-01-01T00:00:00Z2025 Nov2026-05-26T20:07:40.415Z2026-05-26T03:12:06.483ZS-EPMC127302934144008410.3390/brainsci15121286