biostudies-literatureStoyell-Conti FFBLRD VANIDDK NIH HHSNHLBI NIH HHSUnited States Department of Veterans AffairsNIH HHS1998https://www.ebi.ac.uk/biostudies/studies/S-EPMC12731578biostudies-literatureUnknown14(24)The mouse carotid-jugular arteriovenous fistula (AVF) is a widely adopted surgical model to study venous remodeling after AVF creation. Despite its increasing use, the extent to which this model recapitulates the cellular and molecular remodeling processes observed in humans remains uncertain, which is essential for validating its translational relevance. Using bulk and single-cell RNA sequencing, we have depicted the transcriptional and cellular evolution of the mouse jugular vein after AVF anastomosis. Global transcriptomic profiling revealed that venous remodeling begins with a robust inflammatory response, followed by a prominent extracellular matrix (ECM) remodeling phase that peaks at postoperative day 10. Single-cell analyses confirmed the role of macrophage (3-fold) and neutrophil CellsSequential Inflammatory and Matrisome Programs Drive Remodeling of the Mouse Carotid-Jugular Arteriovenous Fistula.PMC12731578I01 BX006080R01 DK132888I01-BX006080IK6-BX0068235F32HL158216-03IK6 BX006823F32 HL1582165R01DK132888-041R01DK144860-01R01 DK136297R01 DK1448605R01DK136297-02Tabbara MYang XVazquez-Padron RIStoyell-Conti FFKaiser AMMartinez LLopez-McCormick JSRojas MGWei YSussman MSfalseSequential Inflammatory and Matrisome Programs Drive Remodeling of the Mouse Carotid-Jugular Arteriovenous Fistula.The mouse carotid-jugular arteriovenous fistula (AVF) is a widely adopted surgical model to study venous remodeling after AVF creation. Despite its increasing use, the extent to which this model recapitulates the cellular and molecular remodeling processes observed in humans remains uncertain, which is essential for validating its translational relevance. Using bulk and single-cell RNA sequencing, we have depicted the transcriptional and cellular evolution of the mouse jugular vein after AVF anastomosis. Global transcriptomic profiling revealed that venous remodeling begins with a robust inflammatory response, followed by a prominent extracellular matrix (ECM) remodeling phase that peaks at postoperative day 10. Single-cell analyses confirmed the role of macrophage (3-fold) and neutrophil 2025-01-01T00:00:00Z2025 Dec2026-05-27T03:17:53.895Z2026-05-27T03:11:46.822ZS-EPMC127315784144001910.3390/cells14241998