biostudies-literatureMa HTKai-Ling WangYong-Zeng ZhangHai-Feng Li978https://www.ebi.ac.uk/biostudies/studies/S-EPMC12731972biostudies-literatureUnknown47(12)<i>Astragalus</i> L. (Fabaceae), the largest plant genus with significant medicinal value, faces critical endangerment of its wild resources and a scarcity of chloroplast genomic data. We sequenced and assembled the complete chloroplast (cp) genomes of four <i>Astragalus</i> species (<i>A. yunnanensis</i>, <i>A. yunnanensis</i> subsp. <i>incanus, A. polycladus</i> and <i>A. polycladus</i> var. <i>nigrescens</i>) and performed comparative analyses with five previously published chloroplast genomes. The cp genomes of the four <i>Astragalus</i> species ranged in size from 122,868 bp to 125,752 bp, all lacking one inverted repeat (IR) region, thus belonging to the inverted repeat lacking clade (IRLC). Annotation revealed that each genome contained 110 unique genes, including 76 protein-coding Current issues in molecular biologyA Comprehensive Comparative Analysis and Phylogenetic Investigation of the Chloroplast Genome Sequences in Four <i>Astragalus</i> Species.PMC12731972Grant No. 202305AF150048Grant No. 202305AG340015Grant Nos. 32300181Grant Nos. 82360708Grant No. 202401CF070015Zhang YZMa HTChen QYRao JHJiang BWang KLfalseA Comprehensive Comparative Analysis and Phylogenetic Investigation of the Chloroplast Genome Sequences in Four <i>Astragalus</i> Species.<i>Astragalus</i> L. (Fabaceae), the largest plant genus with significant medicinal value, faces critical endangerment of its wild resources and a scarcity of chloroplast genomic data. We sequenced and assembled the complete chloroplast (cp) genomes of four <i>Astragalus</i> species (<i>A. yunnanensis</i>, <i>A. yunnanensis</i> subsp. <i>incanus, A. polycladus</i> and <i>A. polycladus</i> var. <i>nigrescens</i>) and performed comparative analyses with five previously published chloroplast genomes. The cp genomes of the four <i>Astragalus</i> species ranged in size from 122,868 bp to 125,752 bp, all lacking one inverted repeat (IR) region, thus belonging to the inverted repeat lacking clade (IRLC). Annotation revealed that each genome contained 110 unique genes, including 76 protein-coding 2025-01-01T00:00:00Z2025 Nov2026-06-19T03:10:42.963Z2026-06-19T03:07:14.459ZS-EPMC127319724161474310.3390/cimb47120978