biostudies-literatureWang BThe Special Project of Shijiazhuang City in Cooperation with the Chinese Academy of Agricultural Sciences11866https://www.ebi.ac.uk/biostudies/studies/S-EPMC12732999biostudies-literatureUnknown26(24)Fusarium stalk rot, which is a common disease caused by <i>Fusarium</i> species, can seriously decrease maize grain yield and quality. Hence, the genetic mechanism mediating maize resistance to Fusarium stalk rot must be elucidated and the associated resistance genes useful for breeding disease-resistant cultivars should be identified. In this study, the highly resistant maize inbred line H1710 and highly susceptible inbred line Huangzaosi were used to construct segregating populations through hybridization, backcrossing, and other methods. A resistance/susceptibility pool was constructed from the F₂ population. A BSA-seq analysis revealed one candidate region associated with stalk rot resistance on chromosome 6; this region (3.98 Mb) contains 38 genes. Furthermore, KASP moleculInternational journal of molecular sciencesGene Mapping and Genetic Analysis of Maize Resistance to Stalk Rot.PMC12732999232490012AXu LDing XLi ZMa LFeng JWang BWang SDuan CCheng ZfalseGene Mapping and Genetic Analysis of Maize Resistance to Stalk Rot.Fusarium stalk rot, which is a common disease caused by <i>Fusarium</i> species, can seriously decrease maize grain yield and quality. Hence, the genetic mechanism mediating maize resistance to Fusarium stalk rot must be elucidated and the associated resistance genes useful for breeding disease-resistant cultivars should be identified. In this study, the highly resistant maize inbred line H1710 and highly susceptible inbred line Huangzaosi were used to construct segregating populations through hybridization, backcrossing, and other methods. A resistance/susceptibility pool was constructed from the F₂ population. A BSA-seq analysis revealed one candidate region associated with stalk rot resistance on chromosome 6; this region (3.98 Mb) contains 38 genes. Furthermore, KASP molecul2025-01-01T00:00:00Z2025 Dec2026-07-05T03:12:29.847Z2026-07-05T03:08:30.537ZS-EPMC127329994146529410.3390/ijms262411866