biostudies-literatureRodriguez-Paez LSECIHTISecretaría de Investigación y Posgrado del Instituto Politécnico Nacional11895https://www.ebi.ac.uk/biostudies/studies/S-EPMC12733172biostudies-literatureUnknown26(24)Autophagy is an intracellular process that recycles and degrades cytoplasmic components, including organelles and macromolecules, to provide energy and basic components for cell survival, maintain cellular homeostasis, and avoid self-damage. It is currently not fully known if mouse sperm undergoes the autophagy process, nor is the subcellular distribution, protein levels of autophagy-related proteins, and the biological role of autophagy in epididymal mouse sperm physiology fully understood. We aimed to investigate key autophagy markers, including LC3 (microtubule-associated protein 1A/1B-light chain 3), p62/SQSTM1 (Sequestosome 1), and mTOR (mechanistic Target of Rapamycin), in epididymal mouse sperm under capacitation (Cap) or non-capacitation (NC) conditions. Furthermore, we evaluated tInternational journal of molecular sciencesAutophagy-Related Proteins Influence Mouse Epididymal Sperm Motility.PMC1273317295426531856630654062025343811644Rodriguez-Paez LMagana JJCordero-Martinez JAguirre-Alvarado CChamorro-Cevallos GRosales-Cruz ECristobal-Luna JMJimenez-Gutierrez GEAlcantara-Farfan VReyes-Maldonado EfalseAutophagy-Related Proteins Influence Mouse Epididymal Sperm Motility.Autophagy is an intracellular process that recycles and degrades cytoplasmic components, including organelles and macromolecules, to provide energy and basic components for cell survival, maintain cellular homeostasis, and avoid self-damage. It is currently not fully known if mouse sperm undergoes the autophagy process, nor is the subcellular distribution, protein levels of autophagy-related proteins, and the biological role of autophagy in epididymal mouse sperm physiology fully understood. We aimed to investigate key autophagy markers, including LC3 (microtubule-associated protein 1A/1B-light chain 3), p62/SQSTM1 (Sequestosome 1), and mTOR (mechanistic Target of Rapamycin), in epididymal mouse sperm under capacitation (Cap) or non-capacitation (NC) conditions. Furthermore, we evaluated t2025-01-01T00:00:00Z2025 Dec2026-06-04T03:20:27.699Z2026-06-04T03:12:26.284ZS-EPMC127331724146532510.3390/ijms262411895