biostudies-literatureFarias-Cea AUniversidad de Santiago de ChileAgencia Nacional de Investigación y DesarrolloUniversidad de La Frontera1807https://www.ebi.ac.uk/biostudies/studies/S-EPMC12735686biostudies-literatureUnknown18(12)<b>Background/Objective:</b> Serotonin and dopamine are key neurotransmitters involved in regulating mood, anxiety, and locomotor activity. Specific transporters mediate their reuptake, SERT and DAT, making them targets for drugs such as Fluoxetine and Methylphenidate. Zebrafish (<i>Danio rerio</i>), due to their genetic and neurochemical similarity to humans, serve as a valuable model for studying the behavioral effects of these drugs. This study aimed to compare the behavioral phenotypes induced by SERT and DAT blockers in adult zebrafish using the Novel Tank Diving Test (NTT), thereby generating a swimming profile for drugs acting on these monoamine transporters that can be utilized in drug discovery and behavior. <b>Methods</b>: Adult zebrafish were administered Fluoxetine or MethylphePharmaceuticals (Basel, Switzerland)Distinct Swimming Behavioral Phenotypes Following Serotonin and Dopamine Transporter Modulation in the Adult Zebrafish Novel Tank Diving Test (NTT).PMC12735686TD23-0016124-0141022401RP124-06882124162821240481Hernandez VBravo JALeal CSotomayor-Zarate RAties-Perez CRojas-Hidalgo FIturriaga-Vasquez PReyes-Parada MJulio-Pieper MSegura KMartinez LMHodar-Salazar MPerez LFarias-Cea ABorroto-Escuela DOfalseDistinct Swimming Behavioral Phenotypes Following Serotonin and Dopamine Transporter Modulation in the Adult Zebrafish Novel Tank Diving Test (NTT).<b>Background/Objective:</b> Serotonin and dopamine are key neurotransmitters involved in regulating mood, anxiety, and locomotor activity. Specific transporters mediate their reuptake, SERT and DAT, making them targets for drugs such as Fluoxetine and Methylphenidate. Zebrafish (<i>Danio rerio</i>), due to their genetic and neurochemical similarity to humans, serve as a valuable model for studying the behavioral effects of these drugs. This study aimed to compare the behavioral phenotypes induced by SERT and DAT blockers in adult zebrafish using the Novel Tank Diving Test (NTT), thereby generating a swimming profile for drugs acting on these monoamine transporters that can be utilized in drug discovery and behavior. <b>Methods</b>: Adult zebrafish were administered Fluoxetine or Methylphe2025-01-01T00:00:00Z2025 Nov2026-07-05T03:12:39.344Z2026-07-05T03:08:19.994ZS-EPMC127356864147129610.3390/ph18121807