biostudies-literatureRuan Jthe Xinjiang Uygur Autonomous Region Science and Technology Program ProjectInnovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine4754https://www.ebi.ac.uk/biostudies/studies/S-EPMC12736087biostudies-literatureUnknown30(24)This study focuses on polysaccharides from <i>Cistanche deserticola</i> and <i>Cistanche tubulosa</i>, medicinal plants renowned for their health benefits. The "water extraction and alcohol precipitation" method was used to obtain the crude polysaccharides of the wine-making residues of <i>C. deserticola</i> (CDP) and <i>C. tubulosa</i> (CTP), respectively. Then, ultrafiltration membrane (UFM), DEAE-52, and Sephadex-G75 or Smartdex-G100 gel chromatography were used to separate and purify the crude polysaccharides, yielding the homogeneous fractions <b>CDP1-5-1</b>, <b>CDP2-2-2</b>, <b>CDP2-3-2</b>, <b>CTP1-5-1</b>, and <b>CTP1-5-3</b>. Structural analysis was conducted by using Fourier-transform infrared spectroscopy (FT-IR), high-performance anion-exchange chromatography coupled with multMolecules (Basel, Switzerland)Comparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of &lt;i&gt;Cistanche&lt;/i&gt;.PMC127360872022LQ04007ZYYCXTD-C-202009Ruan JYu LChen AZhang JZhang YWang DWang TZhang PfalseComparison of Extraction, Isolation, Purification, Structural Characterization and Immunomodulatory Activity of Polysaccharides from Two Species of &lt;i&gt;Cistanche&lt;/i&gt;.This study focuses on polysaccharides from <i>Cistanche deserticola</i> and <i>Cistanche tubulosa</i>, medicinal plants renowned for their health benefits. The "water extraction and alcohol precipitation" method was used to obtain the crude polysaccharides of the wine-making residues of <i>C. deserticola</i> (CDP) and <i>C. tubulosa</i> (CTP), respectively. Then, ultrafiltration membrane (UFM), DEAE-52, and Sephadex-G75 or Smartdex-G100 gel chromatography were used to separate and purify the crude polysaccharides, yielding the homogeneous fractions <b>CDP1-5-1</b>, <b>CDP2-2-2</b>, <b>CDP2-3-2</b>, <b>CTP1-5-1</b>, and <b>CTP1-5-3</b>. Structural analysis was conducted by using Fourier-transform infrared spectroscopy (FT-IR), high-performance anion-exchange chromatography coupled with mult2025-01-01T00:00:00Z2025 Dec2026-05-27T08:03:09.683Z2026-05-27T03:12:10.908ZS-EPMC127360874147178010.3390/molecules30244754