biostudies-literatureHuang YZhejiang Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational College Students' Innovation and Entrepreneurship Training Program of Zhejiang A&F UniversityProgram for Research and Development of Zhejiang A&F University3831https://www.ebi.ac.uk/biostudies/studies/S-EPMC12736802biostudies-literatureUnknown14(24)Glossy appearance is a critical trait that affects the appearance quality and marketability of leafy vegetables, including Chinese cabbage. The glossy trait is primarily associated with cuticular wax. Although several genes involved in cuticular wax biosynthesis have been characterized in Chinese cabbage, the regulatory relationships among them remain unclear. In this study, we identified a glossy mutant, <i>glossy leaf4</i> (<i>gl4</i>), and cuticular wax crystals in the <i>gl4</i> mutant were obviously reduced. Genetic analysis indicated that the glossy phenotype in the <i>gl4</i> mutant appears to be controlled by a single recessive gene. Using a bulked segregant analysis coupled with next-generation sequencing (BSA-seq) and map-based cloning methods, the <i>AtCER2</i> homologous gene <Plants (Basel, Switzerland)Identification of a Leaf Cuticular Wax Biosynthesis Gene <i>BrCER2</i> in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>).PMC1273680232272742LQN25C1500082024LFR070202410341066Xu LFang HWu JHuang YZang YHuang MYing WWang YBai XYang CfalseIdentification of a Leaf Cuticular Wax Biosynthesis Gene <i>BrCER2</i> in Chinese Cabbage (<i>Brassica rapa</i> L. ssp. <i>pekinensis</i>).Glossy appearance is a critical trait that affects the appearance quality and marketability of leafy vegetables, including Chinese cabbage. The glossy trait is primarily associated with cuticular wax. Although several genes involved in cuticular wax biosynthesis have been characterized in Chinese cabbage, the regulatory relationships among them remain unclear. In this study, we identified a glossy mutant, <i>glossy leaf4</i> (<i>gl4</i>), and cuticular wax crystals in the <i>gl4</i> mutant were obviously reduced. Genetic analysis indicated that the glossy phenotype in the <i>gl4</i> mutant appears to be controlled by a single recessive gene. Using a bulked segregant analysis coupled with next-generation sequencing (BSA-seq) and map-based cloning methods, the <i>AtCER2</i> homologous gene <2025-01-01T00:00:00Z2025 Dec2026-05-27T03:15:05.123Z2026-05-27T03:11:23.448ZS-EPMC127368024147071210.3390/plants14243831