biostudies-literatureOtero LMUS Centers for Disease Control and Prevention1539https://www.ebi.ac.uk/biostudies/studies/S-EPMC12737552biostudies-literatureUnknown17(12)<i>Aedes aegypti</i> is the primary urban vector for several important arboviruses, including dengue, chikungunya, yellow fever, and Zika viruses. Traditional dengue virus (DENV) surveillance relies on passive reporting of human cases, which often underestimates transmission due to asymptomatic or unreported infections. This study evaluated the utility of monitoring DENV in <i>Ae. aegypti</i> mosquitoes to improve detection of local dengue transmission and inform vector control strategies during the 2024 dengue epidemic in Puerto Rico. Mosquito surveillance was conducted in 15 neighborhoods within the San Juan metropolitan area where confirmed dengue cases had been recently reported. Adult female <i>Ae. aegypti</i> were collected weekly using Autocidal Gravid Ovitraps (AGO traps) placed wiVirusesMonitoring Dengue Virus in <i>Aedes aegypti</i> to Improve Dengue Surveillance and Control in Puerto Rico.PMC127375520000Adams LMedina JOtero LMTorres JRivera RMaldonado YPaz-Bailey GBarrera RRuiz-Valcarcel JMadewell ZJfalseMonitoring Dengue Virus in <i>Aedes aegypti</i> to Improve Dengue Surveillance and Control in Puerto Rico.<i>Aedes aegypti</i> is the primary urban vector for several important arboviruses, including dengue, chikungunya, yellow fever, and Zika viruses. Traditional dengue virus (DENV) surveillance relies on passive reporting of human cases, which often underestimates transmission due to asymptomatic or unreported infections. This study evaluated the utility of monitoring DENV in <i>Ae. aegypti</i> mosquitoes to improve detection of local dengue transmission and inform vector control strategies during the 2024 dengue epidemic in Puerto Rico. Mosquito surveillance was conducted in 15 neighborhoods within the San Juan metropolitan area where confirmed dengue cases had been recently reported. Adult female <i>Ae. aegypti</i> were collected weekly using Autocidal Gravid Ovitraps (AGO traps) placed wi2025-01-01T00:00:00Z2025 Nov2026-05-27T03:20:22.48Z2026-05-27T03:12:02.376ZS-EPMC127375524147221010.3390/v17121539