biostudies-literatureUnknown16Nataraj VCharcoal rot is a soil- and seed-borne disease caused by a necrotrophic fungal pathogen-<i>Macrophomina phaseolina</i>. To understand the genetic architecture of resistance against it, a genome-wide association study (GWAS) was conducted based on a glasshouse experiment and a 3-year field experiment using 214 diverse soybean accessions. In a glasshouse experiment at the seedling stage, eight single-nucleotide polymorphisms (SNPs) were identified: one SNP each on chromosome (chr) 8 (<i>S8_16817767</i>), chr 10 (<i>S10_52066337</i>), chr 14 (<i>S14_50857981</i>), chr 15 (<i>S15_32620059</i>), chr 17 (<i>S17_1689021</i>), and chr 18 (<i>S18_9413708</i>), while two SNPs (<i>S16_34569104</i> and <i>S16_37878937</i>) were located on chr 16. In the case of the field experiment at the reproductiveFrontiers in plant science1649397https://www.ebi.ac.uk/biostudies/studies/S-EPMC12748003biostudies-literatureUnveiling key genetic determinants of charcoal rot resistance in soybean via genome-wide association studies.PMC12748003Raghuvanshi RGupta SMaranna SSingh KHRajput LSNataraj VPathak KKumawat GMandloi SAmrate PKMohare SShrivastava MKChitikineni ARajesh VRatnaparkhe MBNaik K BAgrawal NVarshney RKfalseUnveiling key genetic determinants of charcoal rot resistance in soybean via genome-wide association studies.Charcoal rot is a soil- and seed-borne disease caused by a necrotrophic fungal pathogen-<i>Macrophomina phaseolina</i>. To understand the genetic architecture of resistance against it, a genome-wide association study (GWAS) was conducted based on a glasshouse experiment and a 3-year field experiment using 214 diverse soybean accessions. In a glasshouse experiment at the seedling stage, eight single-nucleotide polymorphisms (SNPs) were identified: one SNP each on chromosome (chr) 8 (<i>S8_16817767</i>), chr 10 (<i>S10_52066337</i>), chr 14 (<i>S14_50857981</i>), chr 15 (<i>S15_32620059</i>), chr 17 (<i>S17_1689021</i>), and chr 18 (<i>S18_9413708</i>), while two SNPs (<i>S16_34569104</i> and <i>S16_37878937</i>) were located on chr 16. In the case of the field experiment at the reproductive2025-01-01T00:00:00Z20252026-05-28T03:24:27.646Z2026-05-28T03:12:07.752ZS-EPMC127480034147726810.3389/fpls.2025.1649397