biostudies-literatureUnknown54(6)Lin F<h4>Objectives</h4>To investigate the molecular mechanism by which He's Yangchao recipe improves ovarian function in premature ovarian insufficiency (POI) mice through intestinal flora modulation.<h4>Methods</h4>Forty female ICR mice (aged 6-8 weeks) were intraperitoneally injected with cyclophosphamide (150 mg/kg) to establish a POI model, while 10 untreated mice served as the blank control. The cyclophosphamide-injected mice were randomly divided into four groups with 10 mice in each group: low-dose He's Yangchao recipe (crude herb, 6 g/kg), high-dose He's Yangchao recipe (crude herb, 25 g/kg), estradiol (positive control), and model control (distilled water). Treatments were administered daily by gavage for 6 weeks. Vaginal exfoliated cells were stained with Wright-Giemsa solution to moZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences785-793https://www.ebi.ac.uk/biostudies/studies/S-EPMC12750045biostudies-literature[He&lt;b&gt;'&lt;/b&gt;s Yangchao recipe ameliorates premature ovarian insuf-ficiency by remodeling intestinal microbiota to promote granulosa cell glycolysis].PMC12750045Wang RLin FMiao CLiu QChen YZhao YZhang Qfalse[He&lt;b&gt;'&lt;/b&gt;s Yangchao recipe ameliorates premature ovarian insuf-ficiency by remodeling intestinal microbiota to promote granulosa cell glycolysis].<h4>Objectives</h4>To investigate the molecular mechanism by which He's Yangchao recipe improves ovarian function in premature ovarian insufficiency (POI) mice through intestinal flora modulation.<h4>Methods</h4>Forty female ICR mice (aged 6-8 weeks) were intraperitoneally injected with cyclophosphamide (150 mg/kg) to establish a POI model, while 10 untreated mice served as the blank control. The cyclophosphamide-injected mice were randomly divided into four groups with 10 mice in each group: low-dose He's Yangchao recipe (crude herb, 6 g/kg), high-dose He's Yangchao recipe (crude herb, 25 g/kg), estradiol (positive control), and model control (distilled water). Treatments were administered daily by gavage for 6 weeks. Vaginal exfoliated cells were stained with Wright-Giemsa solution to mo2025-01-01T00:00:00Z2025 Dec2026-05-28T03:22:37.537Z2026-05-28T03:12:02.107ZS-EPMC127500454066086810.3724/zdxbyxb-2024-0604