biostudies-literatureUnknown11(1)He R<h4>Purpose</h4>Preclinical studies have demonstrated that FLASH radiation therapy (RT) delivered at ultrahigh-dose rates exerts a preferential normal tissue-sparing effect. This study aimed to delineate the disparities in delayed cognitive function and biological outcomes of whole-brain irradiation in healthy mice, comparing FLASH-RT with conventional RT (CONV-RT).<h4>Methods and materials</h4>Eighty adult male C57BL/6J mice were divided into 5 groups: Sham, CONV-RT10Gy, CONV-RT20Gy, FLASH-RT10Gy, and FLASH-RT20Gy. Whole-brain irradiation was conducted on mice using a miniaturized x-ray FLASH platform at field-average dose rates of 2 Gy/min for CONV-RT and 213 Gy/s for FLASH-RT. Two months after irradiation, we assessed the mice's cognitive function and the number of astrocytes and neuronAdvances in radiation oncology101950https://www.ebi.ac.uk/biostudies/studies/S-EPMC12754397biostudies-literatureThe Impacts of FLASH Radiation Therapy and Conventional Radiation Therapy on the Cognitive Abilities of Mice.PMC12754397Li WLiu XQu BYang YZhang JWu DCheng GHe RDu LWang JXie SfalseThe Impacts of FLASH Radiation Therapy and Conventional Radiation Therapy on the Cognitive Abilities of Mice.<h4>Purpose</h4>Preclinical studies have demonstrated that FLASH radiation therapy (RT) delivered at ultrahigh-dose rates exerts a preferential normal tissue-sparing effect. This study aimed to delineate the disparities in delayed cognitive function and biological outcomes of whole-brain irradiation in healthy mice, comparing FLASH-RT with conventional RT (CONV-RT).<h4>Methods and materials</h4>Eighty adult male C57BL/6J mice were divided into 5 groups: Sham, CONV-RT10Gy, CONV-RT20Gy, FLASH-RT10Gy, and FLASH-RT20Gy. Whole-brain irradiation was conducted on mice using a miniaturized x-ray FLASH platform at field-average dose rates of 2 Gy/min for CONV-RT and 213 Gy/s for FLASH-RT. Two months after irradiation, we assessed the mice's cognitive function and the number of astrocytes and neuron2026-01-01T00:00:00Z2026 Jan2026-06-06T09:24:09.956Z2026-05-28T03:11:34.554ZS-EPMC127543974147988210.1016/j.adro.2025.101950