biostudies-literatureShiu SCHong Kong UniversityNSFResearch Grants Council of Hong KongHKUMinistry of Science and TechnologiesGuangdong Basic and Applied Basic Research FoundationHong Kong Jockey Club Charities TrustHong Kong Special Administrative Region GovernmentMaterials Innovation Institute for Life Sciences and Energygkaf1409https://www.ebi.ac.uk/biostudies/studies/S-EPMC12754780biostudies-literatureUnknown53(22)Assembly of DNA nanostructures to sub-millimetre scales is expected to have significant potential for applications in materials science and medicine. One approach to control nanostructure growth is through using acoustic waves to create pressure nodes for clustering. Here, we report a facet-based underlying DNA nanostructure architecture with structural and stability characteristics ideal for acoustic patterning. The architecture comprises only 16 canonical DNA oligonucleotides which self-assemble to form a nested cube, inspired by the four-dimensional hypercube known as a "tesseract." Cryogenic electron microscopy (Cryo-EM) and atomic force microscopy (AFM) analysis revealed a fully formed tesseract structure with exceptional stiffness and a melting temperature of 84°C, significantly highNucleic acids researchAcoustically seeded fabrication of a DNA tesseract into a conductive wire.PMC12754780STG 3/E-704/23-NGDZX230400332827921817125221102009959T12-201/20-R2023YFF15009001712712417125920CMMI 2323969Whitehouse WLDeLuca MFang NXChui WHLi JCui Jvon Torklus SHKhan AGArya GSiu RHPKinghorn ABYu TFZhang PDong EQu STanner JATse ECMShiu SCHe WAl-Jamal KTMo XZhou KCheung CCLWang XYfalseAcoustically seeded fabrication of a DNA tesseract into a conductive wire.Assembly of DNA nanostructures to sub-millimetre scales is expected to have significant potential for applications in materials science and medicine. One approach to control nanostructure growth is through using acoustic waves to create pressure nodes for clustering. Here, we report a facet-based underlying DNA nanostructure architecture with structural and stability characteristics ideal for acoustic patterning. The architecture comprises only 16 canonical DNA oligonucleotides which self-assemble to form a nested cube, inspired by the four-dimensional hypercube known as a "tesseract." Cryogenic electron microscopy (Cryo-EM) and atomic force microscopy (AFM) analysis revealed a fully formed tesseract structure with exceptional stiffness and a melting temperature of 84°C, significantly high2025-01-01T00:00:00Z2025 Nov2026-06-06T08:30:13.291Z2026-05-27T03:12:28.166ZS-EPMC127547804147426710.1093/nar/gkaf1409