{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Mukharya A"],"pubmed_abstract":["An efficient, accurate, sustainable, and reproducible chromatographic method for Quercetin estimation was developed, optimized, and further validated using a Design of Experiments approach in compliance with ICH guidelines. The Box-Behnken Quality-by-Design optimized critical parameters, yielding robust performance, excellent linearity (R<sup>2</sup> = 0.9996, 0.05-50 μg/mL), and high sensitivity (LOD: 40.27 ng/mL, LOQ: 122.03 ng/mL). Deliberate modifications evaluated by analysis of variance verified the method's significance (P < 0.0001). The method utilises a Phenomenex HyperClone C18 LC Column (250 × 4.6 mm; 5 μm, 130 Å) with an isocratic mobile phase consisting of acidified water (0.1% v/v Triethylamine, pH 3.0 ± 0.05) and Acetonitrile (55:45% v/v). Forced degradation studies revealed"],"journal":["Scientific reports"],"pagination":["2"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12764978"],"repository":["biostudies-literature"],"pubmed_title":["Quality-by-design based RP-HPLC analytical method for the estimation of quercetin in silver-metal organic frameworks (AgMOF): a Box-Behnken optimization approach."],"pmcid":["PMC12764978"],"pubmed_authors":["Mukharya A","Mutalik S"],"additional_accession":[]},"is_claimable":false,"name":"Quality-by-design based RP-HPLC analytical method for the estimation of quercetin in silver-metal organic frameworks (AgMOF): a Box-Behnken optimization approach.","description":"An efficient, accurate, sustainable, and reproducible chromatographic method for Quercetin estimation was developed, optimized, and further validated using a Design of Experiments approach in compliance with ICH guidelines. The Box-Behnken Quality-by-Design optimized critical parameters, yielding robust performance, excellent linearity (R<sup>2</sup> = 0.9996, 0.05-50 μg/mL), and high sensitivity (LOD: 40.27 ng/mL, LOQ: 122.03 ng/mL). Deliberate modifications evaluated by analysis of variance verified the method's significance (P < 0.0001). The method utilises a Phenomenex HyperClone C18 LC Column (250 × 4.6 mm; 5 μm, 130 Å) with an isocratic mobile phase consisting of acidified water (0.1% v/v Triethylamine, pH 3.0 ± 0.05) and Acetonitrile (55:45% v/v). Forced degradation studies revealed","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T10:26:47.006Z","creation":"2026-05-29T03:11:45.844Z"},"accession":"S-EPMC12764978","cross_references":{"pubmed":["41413705"],"doi":["10.1038/s41598-025-32452-6"]}}