biostudies-literatureUnknown9(3)Prabhudesai SEndothelial cells (ECs) in the brain communicate with mural cells to facilitate vascular stability. Platelet-derived growth factor-BB (PDGF-BB)/platelet-derived growth factor receptor-β (PDGFR-β) signaling mechanism at EC-mural cell interface helps stabilize the vasculature. How this paracrine signaling is mediated is not known. Our laboratory studies endothelial cilia, a microtubule-based organelle, and its role in promoting vascular stability. We discovered that brain endothelial cilia are located primarily on the basolateral side, and PDGF-BB is expressed in EC cilium. Thus, we hypothesized that endothelium cilium in conjunction with PDGF-BB on the basolateral side is responsible for mural cell recruitment. In this study, using a combination of zebrafish, mice, and human brain model sysLife science alliancee202503460https://www.ebi.ac.uk/biostudies/studies/S-EPMC12769094biostudies-literatureBrain vascular stability relies on PAK2-cilia-PDGF-BB-HSPGs on basolateral side of endothelium.PMC12769094Yang HZhang FDordick JSYang JGirolamo FPrabhudesai SVirgintino DSpearman ADRarick KRRamchandran RWang LErrede MNeumann TPan AYAlvarez CAThirugnanam KMarzullo ABafti SSong XVanderhoef KfalseBrain vascular stability relies on PAK2-cilia-PDGF-BB-HSPGs on basolateral side of endothelium.Endothelial cells (ECs) in the brain communicate with mural cells to facilitate vascular stability. Platelet-derived growth factor-BB (PDGF-BB)/platelet-derived growth factor receptor-β (PDGFR-β) signaling mechanism at EC-mural cell interface helps stabilize the vasculature. How this paracrine signaling is mediated is not known. Our laboratory studies endothelial cilia, a microtubule-based organelle, and its role in promoting vascular stability. We discovered that brain endothelial cilia are located primarily on the basolateral side, and PDGF-BB is expressed in EC cilium. Thus, we hypothesized that endothelium cilium in conjunction with PDGF-BB on the basolateral side is responsible for mural cell recruitment. In this study, using a combination of zebrafish, mice, and human brain model sys2026-01-01T00:00:00Z2026 Mar2026-06-06T10:56:42.678Z2026-05-29T03:13:03.816ZS-EPMC127690944149079710.26508/lsa.202503460