{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Miljkovic JL"],"funding":["Medical Research Council","Wellcome Trust"],"pagination":["85"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12769671"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["H<sub>2</sub>S reversibly modifies low molecular weight (L<sub>MW</sub>SH) and protein (PrSH) thiols to form persulfides (RSS<sup>-</sup>) and polysulfides (RS(S)<sub>n</sub>S<sup>-</sup>) for antioxidant defence and regulation of activity. However, our understanding of the biological significance of these processes is hampered by our inability to quantify these modifications. We develop a sensitive LC-MS/MS procedure that traps the sulfur atom of H<sub>2</sub>S, and the terminal sulfur atom of RSS<sup>-</sup> and RS(S)<sub>n</sub>S<sup>-</sup> as diagnostic products in biological samples. In parallel, we also trap internal S atoms of RS(S)<sub>n</sub>S<sup>-</sup>, enabling quantification of H<sub>2</sub>S, RSS<sup>-</sup> and RS(S)<sub>n</sub>S<sup>-</sup>. L<sub>MW</sub>S(S)<sub>n</sub>"],"journal":["Nature communications"],"pubmed_title":["Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H&lt;sub&gt;2&lt;/sub&gt;S in biological samples."],"pmcid":["PMC12769671"],"funding_grant_id":["220257/B/20/Z","221604/Z/20/Z","MC_UU_00028/4","110158/Z/15/Z","220257/Z/20/Z"],"pubmed_authors":["Miljkovic JL","Burger N","Krieg T","Hartley RC","Yu CS","Jones SA","Aksentijevic D","Caldwell ST","Warrington S","Lee JJ","Murphy MP","Harkiss AH","James AM"],"additional_accession":[]},"is_claimable":false,"name":"Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides and H&lt;sub&gt;2&lt;/sub&gt;S in biological samples.","description":"H<sub>2</sub>S reversibly modifies low molecular weight (L<sub>MW</sub>SH) and protein (PrSH) thiols to form persulfides (RSS<sup>-</sup>) and polysulfides (RS(S)<sub>n</sub>S<sup>-</sup>) for antioxidant defence and regulation of activity. However, our understanding of the biological significance of these processes is hampered by our inability to quantify these modifications. We develop a sensitive LC-MS/MS procedure that traps the sulfur atom of H<sub>2</sub>S, and the terminal sulfur atom of RSS<sup>-</sup> and RS(S)<sub>n</sub>S<sup>-</sup> as diagnostic products in biological samples. In parallel, we also trap internal S atoms of RS(S)<sub>n</sub>S<sup>-</sup>, enabling quantification of H<sub>2</sub>S, RSS<sup>-</sup> and RS(S)<sub>n</sub>S<sup>-</sup>. L<sub>MW</sub>S(S)<sub>n</sub>","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T10:54:48.622Z","creation":"2026-05-29T03:12:30.488Z"},"accession":"S-EPMC12769671","cross_references":{"pubmed":["41345365"],"doi":["10.1038/s41467-025-66795-5"]}}