biostudies-literatureUnknownLavicky JThe molecular mechanisms controlling the differentiation of stem cells into specialized cells and tissues are enormously complex. Deciphering the mechanisms behind this precisely regulated process is essential not only for understanding ontogenesis but also for interpretations of evolutionary dynamics. A deep understanding of differentiation processes in various cell types would open the way for safe and targeted tissue engineering. This was previously limited by technical constraints, but the recent development of multiomic approaches has overcome these barriers, providing unprecedented insight into the intricate workings of this molecular machinery. Based on single-cell RNA-seq analyses of continuously growing mouse teeth, 4 transcription factors (<i>Etv4</i>, <i>Gsc</i>, <i>Sall1</i>, aJournal of dental research220345251348148https://www.ebi.ac.uk/biostudies/studies/S-EPMC12769936biostudies-literatureUnraveling the Transcription Factor Code of Odontoblast Differentiation.PMC12769936Verner JKompanikova PLavicky JBryja VRakultsev VBarta TKrivanek JLopez MGBohaciakova DRaska JWinchester EWCotney JTuaima HSvandova EChochola VBuchtova MEnglmaier LfalseUnraveling the Transcription Factor Code of Odontoblast Differentiation.The molecular mechanisms controlling the differentiation of stem cells into specialized cells and tissues are enormously complex. Deciphering the mechanisms behind this precisely regulated process is essential not only for understanding ontogenesis but also for interpretations of evolutionary dynamics. A deep understanding of differentiation processes in various cell types would open the way for safe and targeted tissue engineering. This was previously limited by technical constraints, but the recent development of multiomic approaches has overcome these barriers, providing unprecedented insight into the intricate workings of this molecular machinery. Based on single-cell RNA-seq analyses of continuously growing mouse teeth, 4 transcription factors (<i>Etv4</i>, <i>Gsc</i>, <i>Sall1</i>, a2025-01-01T00:00:00Z2025 Jul2026-06-10T04:56:42.243Z2026-06-10T03:07:18.035ZS-EPMC127699364065046510.1177/00220345251348148