biostudies-literatureMaffioli ESapienza Università di RomaUniversità degli Studi di Milano392https://www.ebi.ac.uk/biostudies/studies/S-EPMC12770568biostudies-literatureUnknown16(1)Vision in vertebrates is mediated by the eye, a complex organ with developmental and functional similarities to the central nervous system. Eye proteomics has emerged as a powerful tool for investigating ocular function and disease mechanisms, including neurodegeneration and ocular toxicity. The zebrafish (Danio rerio) is a well-established model in biomedical research, including ophthalmology, due to its highly developed visual system, rapid eye maturation, and genetic homology with humans. Building on previous findings that thermal stress can affect neural tissues, this study investigates whether prolonged exposure to non-optimal temperatures also impacts the zebrafish eye proteome. Adult zebrafish were maintained for 21 days at elevated (34 °C), control (26 °C), or low (18 °C) temperatuScientific reportsChronic environmental temperature affects protein expression in the eye of adult zebrafish (Danio rerio).PMC12770568Progetti di Ricerca RM12117A383FC177APC initiativeFrabetti FToni MMaffioli ENegri AGrassi Scalvini FNonnis SGrana JTedeschi GfalseChronic environmental temperature affects protein expression in the eye of adult zebrafish (Danio rerio).Vision in vertebrates is mediated by the eye, a complex organ with developmental and functional similarities to the central nervous system. Eye proteomics has emerged as a powerful tool for investigating ocular function and disease mechanisms, including neurodegeneration and ocular toxicity. The zebrafish (Danio rerio) is a well-established model in biomedical research, including ophthalmology, due to its highly developed visual system, rapid eye maturation, and genetic homology with humans. Building on previous findings that thermal stress can affect neural tissues, this study investigates whether prolonged exposure to non-optimal temperatures also impacts the zebrafish eye proteome. Adult zebrafish were maintained for 21 days at elevated (34 °C), control (26 °C), or low (18 °C) temperatu2025-01-01T00:00:00Z2025 Nov2026-06-10T03:10:53.216Z2026-06-10T03:07:32.387ZS-EPMC127705684131877810.1038/s41598-025-29745-1