biostudies-literatureUnknown14(12)Huang L<h4>Background</h4>The mechanisms underlying the pronounced organotropism of breast cancer (BC) for the liver remain incompletely elucidated. This study aims to investigate the role of the tumor microenvironment (TME), particularly tumor-associated neutrophils (TANs), and their metabolic regulation in breast cancer liver metastasis (BCLM).<h4>Methods</h4>We employed a multi-level integrative approach. Bioinformatic analysis of Gene Expression Omnibus (GEO) datasets characterized the immune landscape of BCLM. Clinical specimens, and <i>in vitro</i> assays were utilized to define the functional interplay between TANs and BC cells. Metabolomic profiling of patient samples was conducted to characterize metabolic alterations in BCLM.<h4>Results</h4>A neutrophil-enriched TME was identified as a Translational cancer research8949-8964https://www.ebi.ac.uk/biostudies/studies/S-EPMC12776239biostudies-literatureTumor-associated neutrophils drive liver-specific metastatic progression in breast cancer through methionine metabolism mediated by methionine adenosyltransferase II alpha.PMC12776239Ji TWang QZhen SXu DHuang LSun YChen RWang JZhu QZha XfalseTumor-associated neutrophils drive liver-specific metastatic progression in breast cancer through methionine metabolism mediated by methionine adenosyltransferase II alpha.<h4>Background</h4>The mechanisms underlying the pronounced organotropism of breast cancer (BC) for the liver remain incompletely elucidated. This study aims to investigate the role of the tumor microenvironment (TME), particularly tumor-associated neutrophils (TANs), and their metabolic regulation in breast cancer liver metastasis (BCLM).<h4>Methods</h4>We employed a multi-level integrative approach. Bioinformatic analysis of Gene Expression Omnibus (GEO) datasets characterized the immune landscape of BCLM. Clinical specimens, and <i>in vitro</i> assays were utilized to define the functional interplay between TANs and BC cells. Metabolomic profiling of patient samples was conducted to characterize metabolic alterations in BCLM.<h4>Results</h4>A neutrophil-enriched TME was identified as a 2025-01-01T00:00:00Z2025 Dec2026-07-15T06:08:36.54Z2026-06-30T03:21:22.727ZS-EPMC127762394151009610.21037/tcr-2025-aw-2278