{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Wen D"],"funding":["NIDDK NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases"],"pubmed_abstract":["<h4>Background</h4>Testican-2 is a podocyte-derived glycoprotein encoded by SPOCK2. Circulating levels of testican-2 are associated with less glomerulosclerosis and better kidney prognosis, but its biological function in the podocyte is unknown.<h4>Methods</h4>We studied the protective effect of testican-2 on immortalized cultured human podocytes and in mice treated with adriamycin. We used immunoprecipitation-mass spectrometry to identify binding partners of testican-2 and Bio-Layer Interferometry to characterize these protein-protein interactions. Using global and podocyte-specific Spock2 knockout mice, we assessed the impact of testican-2 deficiency in models of podocyte injury and also tested whether exogenous testican-2 confers podocyte protection in testican-2 deficient mice. Finally"],"journal":["Journal of the American Society of Nephrology : JASN"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12776367"],"repository":["biostudies-literature"],"pubmed_title":["Testican-2 Interaction with the Extracellular Matrix and Podocyte Protection."],"pmcid":["PMC12776367"],"funding_grant_id":["R01 DK108803","R01DK108803","R01DK088327","K08 DK132411","P30 DK057521","K08DK132411","R01 DK088327","P30 DK043351"],"pubmed_authors":["Waikar SS","Rhee EP","Pollak MR","Arnaout MA","Grams ME","Zhou W","Schmidt IM","Rosales IA","Zhang Q","Parada XV","Kim T","van Agthoven J","Weins A","Colvin RB","Wen D"],"additional_accession":[]},"is_claimable":false,"name":"Testican-2 Interaction with the Extracellular Matrix and Podocyte Protection.","description":"<h4>Background</h4>Testican-2 is a podocyte-derived glycoprotein encoded by SPOCK2. Circulating levels of testican-2 are associated with less glomerulosclerosis and better kidney prognosis, but its biological function in the podocyte is unknown.<h4>Methods</h4>We studied the protective effect of testican-2 on immortalized cultured human podocytes and in mice treated with adriamycin. We used immunoprecipitation-mass spectrometry to identify binding partners of testican-2 and Bio-Layer Interferometry to characterize these protein-protein interactions. Using global and podocyte-specific Spock2 knockout mice, we assessed the impact of testican-2 deficiency in models of podocyte injury and also tested whether exogenous testican-2 confers podocyte protection in testican-2 deficient mice. Finally","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-06T12:32:08.681Z","creation":"2026-05-30T03:11:40.35Z"},"accession":"S-EPMC12776367","cross_references":{"pubmed":["41177530"],"doi":["10.1681/asn.0000000915","10.1681/ASN.0000000915"]}}