{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Arp NL"],"funding":["NIAID NIH HHS","NHLBI NIH HHS","NCI NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Nitric oxide (NO•) is an important signaling molecule in many biological processes, including immune response. During response to classical activation stimuli lipopolysaccharide (LPS) and interferon-γ (IFNγ), macrophages generate NO• via inducible nitric oxide synthase (iNOS). To comprehensively define the effects of NO•, we applied a multi-omic strategy integrating proteomics and transcriptomics to profile murine macrophages across conditions with or without LPS/IFNγ-activation, with or without iNOS expression or exogenous NO• donor treatment. The results revealed NO• has broad, yet selected and controlled, regulatory effects, playing a key role in coordinating the systematic remodeling during macrophage classical activation. Among the proteins that are most suppressed in a NO•-dependent "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.12.31.697202"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12776429"],"repository":["biostudies-literature"],"pubmed_title":["Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1."],"pmcid":["PMC12776429"],"funding_grant_id":["R35 GM147014","T32 GM140935","F30 AI183563","T32 GM135066","P41 GM108538","R35 GM118027","F30 HL174128","P30 CA014520"],"pubmed_authors":["Votava JA","Huttenlocher A","Urquiza US","Morgenstern M","Fan J","Arp NL","John SV","Stevens JJ","Schrope JH","Coon JJ"],"additional_accession":[]},"is_claimable":false,"name":"Multi-omic analysis reveals nitric oxide dependent remodeling in classically activated macrophages and identifies negative regulation mediated by AKR1A1.","description":"Nitric oxide (NO•) is an important signaling molecule in many biological processes, including immune response. During response to classical activation stimuli lipopolysaccharide (LPS) and interferon-γ (IFNγ), macrophages generate NO• via inducible nitric oxide synthase (iNOS). To comprehensively define the effects of NO•, we applied a multi-omic strategy integrating proteomics and transcriptomics to profile murine macrophages across conditions with or without LPS/IFNγ-activation, with or without iNOS expression or exogenous NO• donor treatment. The results revealed NO• has broad, yet selected and controlled, regulatory effects, playing a key role in coordinating the systematic remodeling during macrophage classical activation. Among the proteins that are most suppressed in a NO•-dependent ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-12T03:14:58.063Z","creation":"2026-06-12T03:08:14.619Z"},"accession":"S-EPMC12776429","cross_references":{"pubmed":["41509239"],"doi":["10.64898/2025.12.31.697202"]}}