biostudies-literatureUnknown11(1)Zhao LThis study reports the first complete mitochondrial genome of <i>Opsariichthys jishuiensis</i>. The circular genome (16,614 bp) contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. Phylogenetic analysis using Bayesian inference and maximum-likelihood methods identifies <i>O. jishuiensis</i> as most closely related to <i>O. acutipinnis</i> and <i>O. evolans</i>, forming a monophyletic clade within the subfamily Xenocyprinae. These findings confirm the taxonomic placement of <i>Opsariichthys</i> and provide essential genetic resources for the evolutionary biology, taxonomy, and conservation of freshwater fishes in the Huanghe-Huaihe River Basin.Mitochondrial DNA. Part B, Resources216-221https://www.ebi.ac.uk/biostudies/studies/S-EPMC12777922biostudies-literatureCharacterization of the complete mitochondrial genome sequence of &lt;i&gt;Opsariichthys jishuiensis&lt;/i&gt; (Zhao et al., 2025) and phylogenetic analysis.PMC12777922Zhang WLiu JSu CZhao LCuo XTan CYang TYao GfalseCharacterization of the complete mitochondrial genome sequence of &lt;i&gt;Opsariichthys jishuiensis&lt;/i&gt; (Zhao et al., 2025) and phylogenetic analysis.This study reports the first complete mitochondrial genome of <i>Opsariichthys jishuiensis</i>. The circular genome (16,614 bp) contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a control region. Phylogenetic analysis using Bayesian inference and maximum-likelihood methods identifies <i>O. jishuiensis</i> as most closely related to <i>O. acutipinnis</i> and <i>O. evolans</i>, forming a monophyletic clade within the subfamily Xenocyprinae. These findings confirm the taxonomic placement of <i>Opsariichthys</i> and provide essential genetic resources for the evolutionary biology, taxonomy, and conservation of freshwater fishes in the Huanghe-Huaihe River Basin.2026-01-01T00:00:00Z20262026-05-30T03:18:18Z2026-05-30T03:11:38.22ZS-EPMC127779224150901010.1080/23802359.2025.2602218