biostudies-literatureVirk NANorthern Borders Universitye0337642https://www.ebi.ac.uk/biostudies/studies/S-EPMC12779149biostudies-literatureUnknown21(1)Synthetic chemistry facing little difficulties in C-C, C-S, and C-N double bonds activation is a very long-standing challenge. The current studies based on the development of C-N double and single bond, N-N bond and C-S in the trizole and azinane merged rings into single unit. The targeted compounds were obtained through metal free, microwave assisted and conventional techniques. The present work covers sulfonamide, hydrazide synthesis, 1,2,4-triazole, and thioether synthesis to produce piperidine-based triazole analogues (7a-j). An array of electrophiles (6a-j) was treated with 1,2,4-triazole (5) under conventional (59-72% yield) and microwave assisted (87-95% yield) protocols to acquire the targeted molecules (7a-j). The proton and carbon NMR, and IR techniques were used for structural cPloS oneStructural engineering, BSA binding and computational analysis of isonipacotate based enzyme inhibitors containing 1,2,4-triazole.PMC12779149NBU-CRP-2025-2042Iqbal JRehman ZUAlzahrani ARKhan AChohan TAVirk NAAziz-Ur-RehmanJawaid TfalseStructural engineering, BSA binding and computational analysis of isonipacotate based enzyme inhibitors containing 1,2,4-triazole.Synthetic chemistry facing little difficulties in C-C, C-S, and C-N double bonds activation is a very long-standing challenge. The current studies based on the development of C-N double and single bond, N-N bond and C-S in the trizole and azinane merged rings into single unit. The targeted compounds were obtained through metal free, microwave assisted and conventional techniques. The present work covers sulfonamide, hydrazide synthesis, 1,2,4-triazole, and thioether synthesis to produce piperidine-based triazole analogues (7a-j). An array of electrophiles (6a-j) was treated with 1,2,4-triazole (5) under conventional (59-72% yield) and microwave assisted (87-95% yield) protocols to acquire the targeted molecules (7a-j). The proton and carbon NMR, and IR techniques were used for structural c2026-01-01T00:00:00Z20262026-06-06T11:54:14.734Z2026-05-30T03:08:43.274ZS-EPMC127791494149958510.1371/journal.pone.0337642