biostudies-literatureLatzko LTiroler Wissenschaftsförderung134-143https://www.ebi.ac.uk/biostudies/studies/S-EPMC12779247biostudies-literatureUnknown29(1)<h4>Objective</h4>The NLRP3 inflammasome is a damage sensor driving pyroptotic cell death during orthodontic tooth movement (OTM). Recent studies have revealed impaired one-week OTM in mice lacking NLRP3. However, the relevance of NLRP3 during long-term OTM remains unknown.<h4>Materials and methods</h4>OTM was analysed after 7, 14 and 21 days of force application in mice lacking NLRP3 (NLRP3^-/-), wild-type (WT) male and female littermates. Micro-computed tomography (μCT) was used to assess molar movement and bone quality. Tartrate-resistant acid phosphatase (TRAP) staining was used to visualise osteoclastic activity.<h4>Results</h4>We show here that after 2 weeks of force application, OTM was delayed in mice lacking NLRP3 compared to WT mice. Interestingly, OTM regained speed inOrthodontics & craniofacial researchThe NLRP3 Inflammasome Regulates Orthodontic Tooth Movement.PMC12779247F.45069/8-2022F.45069/8‐2022Gruber RHayback JManzl CLatzko LDegenhart GCrismani AGBlumer MJfalseThe NLRP3 Inflammasome Regulates Orthodontic Tooth Movement.<h4>Objective</h4>The NLRP3 inflammasome is a damage sensor driving pyroptotic cell death during orthodontic tooth movement (OTM). Recent studies have revealed impaired one-week OTM in mice lacking NLRP3. However, the relevance of NLRP3 during long-term OTM remains unknown.<h4>Materials and methods</h4>OTM was analysed after 7, 14 and 21 days of force application in mice lacking NLRP3 (NLRP3^-/-), wild-type (WT) male and female littermates. Micro-computed tomography (μCT) was used to assess molar movement and bone quality. Tartrate-resistant acid phosphatase (TRAP) staining was used to visualise osteoclastic activity.<h4>Results</h4>We show here that after 2 weeks of force application, OTM was delayed in mice lacking NLRP3 compared to WT mice. Interestingly, OTM regained speed in2026-01-01T00:00:00Z2026 Feb2026-06-06T11:30:55.967Z2026-05-29T03:12:53.976ZS-EPMC127792474122198110.1111/ocr.70059